梅毒血清学检测流程的临床流行病学评价  

Evaluation on examination process of syphilis serological detection

作　　者：彭红[1] 刘熔[1] 赵素元[1] 

机构地区：[1]新疆维吾尔自治区人民医院临床医学研究中心,新疆维吾尔830001

出　　处：《中国卫生检验杂志》2010年第2期329-331,共3页Chinese Journal of Health Laboratory Technology

摘　　要：目的:对梅毒血清学检测流程进行临床流行病学初步评价。方法:同步进行RPR、ELISA和TPPA检测并就其进行多种组合试验。结果:准确性评价显示灵敏度(RPR+ELISA)>(RPR+TPPA)>ELISA/TPPA>RPR,特异性均高于97%;可靠性评价显示诊断效率ELISA/TPPA/(RPR+TPPA)>(RPR+ELISA)>RPR;有效性评价显示阳性预测值均高于99%,阴性预测值(RPR+ELlSA)>ELISA>TPPA/(RPR+TPPA)>RPR。结论:综合分析显示同步进行RPR和ELISA初筛实验,任一试验呈阳性反应时再行TPPA确认,是目前梅毒筛查最经济、最有效、最理想的方案。Objective：To evaluate the examination process of Syphilis serological test.Methods：Rapid plasma regain test（RPR）,syphilis enzyme linked immunosorbent assay（TP-ELISA）,the T.pallidum particle agglutination test（TPPA） assay were performed in 141 patients and 45 healthy persons to evaluate the accuracy and efficacy of the three assay.Results：Accuracy evaluation displayed that the sensitivity of the positive samples were（RPR＋ELISA）〉（RPR＋TPPA）〉ELISA 〉TPPA 〉RPR,the specificity 〉97% in all assays.Efficacy evaluation displayed that the positive predicting value were higher than 99 % in all assay,and the negative predicting value were（RPR＋ELISA） 〉ELISA〉TPPA〉（RPR＋TPPA）〉RPR.Conclusion：RPR＋ELISA combined detection as preliminary screening assay,and TPPA as a confirm assay when positive case detected in RPR or ELISA,this way maybe the proper one for the examination process of syphilis serological detection at present.

关 键 词：梅毒 血清学检测 流程 评价 

分 类 号：R446.11[医药卫生—诊断学]