三株以亚硝酸氮为氮源的异养硝化菌的分离与分子生物学鉴定  

ISOLATION AND IDENTIFICATION OF THREE STRAINS OF HETEROTROPHIC NITRIFIER WHICH NITROGEN SOURCE WAS NITRITE NITROGEN

作　　者：孔强[1] 付荣恕[1] 任宗明[2] 

机构地区：[1]山东师范大学生命科学学院,济南250014 [2]中国科学院烟台海岸带可持续发展研究所,山东烟台264000

出　　处：《山东师范大学学报（自然科学版）》2010年第1期141-143,146,共4页Journal of Shandong Normal University(Natural Science)

基　　金：国家水体污染控制与治理科技重大专项海河流域水污染综合治理与水质改善技术与集成示范项目（2008ZX07209-006）

摘　　要：从天津北运河沉积物中分离得到3株以亚硝酸氮（NOr—N）为氮源的异养硝化菌HN4、HN5和HN6．对3菌株降解NOr—N与总有机碳（TOC）能力进行了研究．结果表明，3菌株富集培养期内降解NOr—N与TOC效率均在60％以上，富集培养期间NOr—N含量变化和TOC含量变化高度相关，3株异养硝化菌能在进行脱氮作用的同时利用有机碳．以细菌16SrDNA序列和真菌ITS序列进行分子生物学鉴定，初步认定HN4为Shigella，HN5和HN6为Candida palmioleophila．Three heterotrophic nitrifier HN4,HN5 和 HN6 were isolated from the sediments of the Beiyun river in Tianjin, whose nitrogen source was NO2^- -N. The degradation capability of the three heterotrophic nitrifier on NO2^- -N and TOC were investigated. The results showed that these three strains can quickly decrease more than 60% NO2^- - N and TOC in enriched culture medium, the content of NO2^- - N and TOC changes in enriched culture medium were highly related, the denitrification could occur by the strains when they utilized organice. Based on sequence analysis of the bacterial 16S rDNA and fungal ITS sequence, HN4 was initial identified as ShigeUa, and HN5 and HN6 were initial identified as Candida palmioleophila.

关 键 词：异养硝化 16S RDNA序列 ITS序列 

分 类 号：X522[环境科学与工程—环境工程]