用叶绿体trnH-psbA序列分析石杉科植物系统发育和植物条形码的初探  

The Utility of trnH-psbA Gene for Phylogenetic Analysis of Huperziaceae and Plant Barcoding

作　　者：邵浩[1] 张丽[1] 吕会芳[1] 周吉燕[1] 胡之璧[1] 黎万奎[1] 

机构地区：[1]上海中医药大学中药研究所/上海市复方中药重点实验室,上海201203

出　　处：《中药材》2010年第1期18-21,共4页Journal of Chinese Medicinal Materials

基　　金：国家“十一五”科技支撑计划（2006BAI06A16）; 上海市科委中药现代化专项（07DZ19723）; 上海市复方中药重点实验室开放课题基金（09DZ2270900）

摘　　要：目的：研究石杉科17种植物的系统发育关系和石杉科植物条形码。方法：使用叶绿体trnH-psbA基因序列构建了石杉科的系统发育树。结果：表明石杉科植物高度聚类为两个分支,自展支持率达91%。结论：支持Hol-ub和秦仁昌将石杉科分为石杉属和马尾杉属的分类学观点。17种石杉科植物种具有良好的单系性,trnH-psbA基因能在种水平很好区分石杉科植物,可以作为本科分类鉴定条形码。Objective：The purpose of study was to discover the phylogenetic relations and plant barcoding of 17 plants from Huperziaceae.Methods：Phylogenetic tree of chloroplast trnH-psbA gene of 17 plants from Huperziaceae was constructed by software.Results：It showed that Huperziaceae could be divided into two genera Huperzia and Phlegmariurus and bootstrap value reached 91%.Conclusions：Holub and Qing′ taxonomy was supported and 17 species in Huperziaceae were monophyletic groups and it suggested that trnH-psbA could be used as a DNA barcode to identify plants.

关 键 词：TRNH-PSBA 石杉属 植物条形码 

分 类 号：Q75[生物学—分子生物学]