美味猕猴桃茎的HPLC指纹图谱研究  

Study on Fingerprints of the Stems of Actinidia deliciosa by HPLC

作　　者：甄汉深[1] 丘琴[1] 梁洁[1] 葛静[2] 

机构地区：[1]广西中医学院药学院,广西南宁530001 [2]桂林医学院附属医院药剂科,广西桂林541001

出　　处：《中药材》2010年第1期36-40,共5页Journal of Chinese Medicinal Materials

基　　金：广西高校人才小高地建设创新团队资助计划;国家自然科学基金地区联合资助项目(30160095);广西教育厅科研项目(200810LX107);广西中医学院高层次人才科研启动基金课题(G2006047)

摘　　要：目的:建立美味猕猴桃茎的HPLC指纹图谱。方法:采用HPLC法,色谱柱Thermo Hypersil BDS C18柱(250 mm×4.6 mm,5μm),流动相以乙腈-0.1%磷酸溶液梯度洗脱,柱温20℃,检测波长296 nm,流速1.0 mL/min,进样量20μL。结果:建立了美味猕猴桃茎药材的指纹图谱,并检测了不同产地16批美味猕猴桃茎药材,标定了28个共有峰,相似度较高,各色谱峰分离较好,达到中药指纹图谱研究的技术要求。结论:该方法准确可靠,为更好地控制药材内在质量提供了科学依据。Objective： To establish the fingerprint spectrum of the stems of Actinidia deliciosa by HPLC.Methods： HPLC fingerprint analysis of the stems of Actinidia deliciosa was developed and the thermo Hypersil BDS C18 column（250 mm×4.6 mm,5 μm）was used.The mobile phase consisted of Acetonitril-0.1% phosphoric acid with gradient elution.The column temperature was 20℃,the detective wavelength was 296 nm,the flow rate was 1.0 mL/min,and the sample injection was 20 μL.Results： Fingerprint spectrum of the stems of Actinidia deliciosa was established,and 16 samples of different origin Actinidia deliciosa were detected.Twenty-eight peaks in chromatogram were common.There was a high similarity and each chromatographic peak was obtained with good separation correlation according to the technical requirements of fingerprint of Chinese traditional medicine.Conclusion： This method is accurate,reliable and provides a scientific basis for controlling the quality of the stems of Actinidia deliciosa.

关 键 词：美味猕猴桃茎 HPLC 指纹图谱 

分 类 号：R282.5[医药卫生—中药学]