半边旗提取物5F对HepG2细胞凋亡的影响及其机制  

Apoptosis Effect and Mechanism of 5F from Pteris semipinnata on HepG2 Cells

作　　者：李立[1] 刘义[1] 吕应年[1] 吴科锋[1] 陈功[2] 梁念慈[1,3] 

机构地区：[1]广东医学院广东天然药物研究与开发重点实验室,广东湛江524023 [2]香港中文大学韦尔斯亲王医院外科学系 [3]广东医学院生物化学与分子生物学研究所,广东湛江524023

出　　处：《中药材》2010年第1期77-80,共4页Journal of Chinese Medicinal Materials

基　　金：国家自然科学基金(3987099);香港特别行政区政府创新科技基金(GHP/022/06)

摘　　要：目的：研究半边旗（PsL）提取物Ent-11α-hydroxy-15-oxo-kaur-16-en-19-oic-acid（5F）诱导的HepG2细胞凋亡及其可能机制。方法：通过MTT法检测细胞活性以研究HepG2细胞经不同浓度（5-80 mg/L）5F处理24 h所导致的5F细胞毒作用。采用Hoechst/PI实验定性分析细胞凋亡。采用Western blotting实验检测经5F处理的HepG2细胞线粒体内Bax水平及分析cyto-c与AIF在胞浆内的水平。结果：通过细胞活性分析证明,5F对HepG2细胞的细胞毒性随着5F浓度的提高而增强。HepG2经5F处理后,可观察到以细胞核凝聚为特征的凋亡细胞。经5F处理的HepG2细胞线粒体内Bax水平提高,胞浆内cyto-c及AIF水平增强。结论：5F介导的细胞凋亡涉及线粒体依赖途径,5F可能对人类癌细胞具有诱导凋亡作用,尤其是肝细胞癌（HCC）细胞。Objective：To investigate the effect of Pteris semipinnata L.（PsL）extract Ent-11α-hydroxy-15-oxo-kaur-16-en-19-oic-acid（5F）-on HepG2 cells and explore its potential mechanism.Methods：Cytotoxicity of 5F was studied in HepG2 cells treated with different doses of 5F（0～80 mg/L）for 24 h and cell viability was determined by MTT assay.To analyze apoptosis qualitatively,the Hoechst/PI assay was used.The level of Bax in mitochondria fraction of 5F-treated HepG2 cells was determined by western blotting.The levels of cyto-c and AIF in the cytosol were analyzed by western blotting.Results：The cytotoxicity of 5F on HepG2 cells was elevated with the increasing of 5F concentrations,as evidenced by the cell viability assay.The apoptotic cells characterized by condensed neclei were observed after the exposure of HepG2 cells to 5F.The level of Bax in mitochondria fraction of 5F-treated HepG2 cells increased.The levels of cyto-c and AIF in the cytosol of 5F-treated HepG2 cells increased.Conclusion：5F mediated apoptosis involves mitochondria-dependent pathway and 5F might have a therapeutic value against human cancer cell lines and especially on hepatocellular carcinoma（HCC）cells.

关 键 词：半边旗 细胞凋亡 HEPG2细胞 线粒体依赖途径 

分 类 号：R285.5[医药卫生—中药学]