油菜籽原花青素的超声辅助提取  

Extraction of Rape Seed Proanthocyanidin with Ultrasonic Wave

作　　者：张小军[1] 吴建铭[1] 夏春镗[1] 祝建[1] 谢正荣[2] 

机构地区：[1]同济大学生命科学与技术学院,上海200092 [2]昆山市农业局,江苏昆山215300

出　　处：《中药材》2010年第1期114-118,共5页Journal of Chinese Medicinal Materials

基　　金：江苏省科技厅农业重点攻关项目(BE2006310)

摘　　要：通过超声辅助技术对油菜籽原花青素物质进行了提取的单因素试验和正交试验研究,验证了油菜籽原花青素的存在并得出影响提取效果各因素的主次顺序依次为:乙醇浓度>提取温度>超声作用时间>料液比。确定最佳的超声提取条件为:在60℃下,按1∶20(g/mL)的料液比加入浓度为60%乙醇溶液,超声提取20 m in,共3次。按此条件制备得到的原花青素提取率较传统方法提取的原花青素高84.5%。本实验指出,油菜籽作为一种大宗资源,其原花青素含量十分可观。Rape seed proanthrocyanidin was extracted by ultrasonic technology,and the conditions of extraction were studied.The results showed that the order of the factors which influenced the extraction effects were as follows： ethanol concentration,extracting temperature,ultrasonic extracting time and the ratio of the quantity of raw material to extraction agent（w/v）.The optimal conditions were as follows： temperature： 60 ℃,extracting agent： 60% ethanol aqueous,the ratio of the quantity of raw material to extracting agent： 1∶20（w/v）,extracting number：3 times,extracting time：20 min,respectively.The yield of proanthrocyanidins extracted by ultrasonic technology was 84.5% higher than tradional method.Rape Seed was rich in proanthrocyanidin.

关 键 词：油菜籽 原花青素 超声提取 提取率 

分 类 号：R284.2[医药卫生—中药学]