利用碱性磷酸酶抗碱性磷酸酶法检测凝血酶受体  

Detection of Thrombin Receptor by Alkaline Phosphatase Antialkaline Phosphatase Method

作　　者：孙成文[1] 王汉斌[1] 史寅奎[1] 刘龙斌 王海涛[3] 陈万荣[3] 

机构地区：[1]军事医学科学院附属医院心血管病研究室,北京100039 [2]麻省大学医学院血管生物学实验室,美国ma01655 [3]军事医学科学院微生物流行病研究所,北京100085

出　　处：《中国动脉硬化杂志》1998年第4期345-347,共3页Chinese Journal of Arteriosclerosis

摘　　要：凝血酶受体被凝血酶激活时，凝血酶受体结合于细胞膜处氨基末端精氨酸41／丝氨酸42被裂解，释放出一个包含有41个氨基酸残基的多肽（TR1－41）。根据TR1－41的氨基酸序列，对其进行合成，并对小鼠进行免疫，筛选得到了4个产生抗TR1－41单克隆抗体的细胞株。利用抗TR1-41单克隆抗体建立了一种检测凝血酶受休的免疫酶组织化学方法-碱性磷酸酶抗碱性磷酸酶法，并成功地检测了心肌细胞凝血酶受体，该方法可以用于组织器官凝血酶受体的检测。Aim To establish a immunocytochemistry method of detection to thrombin receptor(TR).Methods TR activated by thrombin can release a peptide that contain 41 amino acids(TR1-41)from N-terminal of TR.The TR1-41 was synthesized accord-ing to the amino acids sequence of TR,then mice were immunized utilizing the TR1-41,4 cell strains produc-ing TR-McAb were gotten.Preparations of atheroscle-rotic human left ventricular myocardium of four died patients were cut out.The preparations were made in-to paraffin sections.Results A immunocytochemistry method-alkaline phosphatase antialkaline phosphatase(APAAP)to de-tect the thrombin receptor was established and the TR of myocardium were detected successfully.Conclusion The method established can detect TR expression of tissue and organ.

关 键 词：凝血酶 凝血酶受体 单克隆抗体 测定 

分 类 号：R446.112[医药卫生—诊断学]