体复康对运动性疲劳大鼠脑组织中阿黑皮素原基因表达动态变化的影响  

Dynamic Effect of Tifukang on Brain Proopiomelanocortin Gene Expression of Exercise Induced Fatigue Rat

作　　者：陈家旭[1] 杨维益[1] 梁嵘[1] 黄启福[1] 刘晓兰[1] 董浩[1] 李峰[1] 王天芳[1] 康纯洁[1] 

机构地区：[1]北京中医药大学,北京100029

出　　处：《中国中西医结合杂志》1998年第12期733-736,共4页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：国家自然科学基金重点项目!(No .39430 140 )

摘　　要：目的 :从基因转录水平阐明运动性疲劳产生及药物调节的机理。方法 :采用生物素标记阿黑皮素原 (POMC)cRNA探针 (Bio cRNA)原位杂交法结合图像分析 ,观察中药复方体复康对运动性疲劳大鼠不同脑区POMCmRNA表达。结果 :对于慢性运动应激 ,体复康能增强POMC在海马CA1 区、下丘脑部位的基因表达 ,而能降低POMC在额叶皮层的基因表达。长时期 (共 7周 )大强度的跑台运动 (速度由 1 5m/min递增至 3 5m/min ,运动时间为每天 2 0～2 5min)的大鼠 ,末次运动后POMC基因表达在额叶皮层、海马CA1 下降 (前者至运动结束后 3h仍未恢复 ,后者至运动结束后 3 0min即恢复 ) ,在下丘脑则增强 ;体复康能增强末次运动后POMC在额叶皮层、海马CA1 区、下丘脑等脑区的基因表达 ,尤其是在运动结束后 3h最为显著。结论 :POMCmRNA表达在运动性疲劳大鼠不同脑区呈现不同的动态变化趋势 ,体复康对其POMCmRNA表达有重要动态调节作用。Objective: To study the mechanism of exercise induced fatigue (EIF) and effect of Tifukang (TFK) from the gene transcription level. Methods: In situ hybridization was adopted, using biotin labelled proopiomelanocortin (POMC) cRNA probe and integrating with image pattern analysis, to observe the effect of TFK on changes of POMC mRNA expression in different areas of EIF rat′s brain. Results: TFK could significantly strengthen POMC mRNA expression in hippocampal CA 1 and hypothalamus and inhibit obviously that in frontal cortex of chronic fatigue rats. In rats managed with long term (7 weeks in total) and high intensity exercise with the speed increased from 15 m/min gradually to 35 m/min, 20 to 25 min/d, after the final exercise, the POMC mRNA expression in frontal cortex and hippocampal CA 1 decreased (the latter recovered at 30 minutes after ending exercise but the former was not recovered yet 3 hours after the ending of exercise), while that in hypthalamus increased. TFK could remarkably increase POMC gene expression in the above mentioned three areas after the final exercise especially prominent at the time of 3 hours after the ending of exercise.Conclusion: The dynamic changes of POMC gene expression in different areas of EIF rat′s brain were not the same, and TFK displayed an important dynamic regulating effect on it.

关 键 词：体复康 运动性疲劳 信使核糖核酸 原位杂交 

分 类 号：R286[医药卫生—中药学] R285.5[医药卫生—中医学]