两种方法测定ASO、C4结果的可比性研究  

Comparability analysis of tow methods of immunoturdidimetry on detection of ASO and C4

作　　者：李若林[1] 邓雪莲[2] 徐明辉[1] 李泰阶[1] 李山[1] 秦雪[1] 

机构地区：[1]广西医科大学附属第一医院临床医学实验部,南宁530021 [2]广西医科大学2003级医学检验系,南宁530000

出　　处：《国际检验医学杂志》2010年第2期105-106,共2页International Journal of Laboratory Medicine

基　　金：广西卫生厅科研课题(Z2008158)

摘　　要：目的探讨透射免疫比浊法与乳胶增强散射免疫比浊法测定ASO、C4定量结果的可比性。方法以日立7170全自动生化分析仪5点定标透射免疫比浊法为参比方法(X),德灵BU prospec特定蛋白分析仪的乳胶增强散射免疫比浊法为待评方法(Y),分别测定高、中值ASO血清各12例,高、中值C4血清各13例。结果经分别平行测定不同水平ASO、C4的混合血清各20次,乳胶增强散射比浊法和透射免疫比浊法的检测精密度均符合实验室要求(<5%),但前者的重复性(批内变异CV值均小于2%)较后者(CV均小于3%)好;两法分别测定高、中值ASO和高、中值C4定量结果,差异均有统计学意义(P<0.01),对高、中值ASO和中值C4的相关系数(r)分别为0.982、0.958、0.945,说明两法的相关性良好。而在高值C4两法的相关系数(r)则为0.888。结论乳胶增强散射比浊测定法检测重复性好,灵敏度高,检测范围宽,是一种值得推广应用的较好生化检测方法。Objective To compare ASO and C4 results from differences between the transmission of immune Turbidimetry latex and enhance immune Turbidimetry scattering. Methods Taking HITACHI 7170 automatic biochemical analyzer with five points calibration as the standard method（X） and Germany Ling BN protein analytical system as the detecting method（Y）, 12 sam ples of high,medium level serum ASO and 13 samples of high,medium level serum C4 were measured. The collected data were processed by statistics analysis. Results The precision of the transmission of immune Turbidimetry latex and enhance immune Turbidimetry scattering are in line with the requirements of laboratories by testing parallel to the different levels of ASO,C4 mixed the serum 20. But the former is better. The result were significantly different checked by two methods in the high and medium levels of the ASO and C4（P〈0.01） ,the correlation factors were 0. 982.0. 958.0. 888 and 0. 945 respectively. Conclusion Scattering turbidimetry method showed great reproducibility,higher sensitivity, wider detect range, worthing application and generalization.

关 键 词：散射测浊法和比浊法 实验室技术和方法 对比研究 

分 类 号：R446.6[医药卫生—诊断学]