丙咪嗪对人血小板胞浆游离钙的影响  

Effect of Imipramine on the Cytoplasmic Free Calcium in the Platelets of Human

作　　者：付润芳[1,2] 贾丹辉[1,2] 吴红霞[1,2] 岳京丽[1,2] 可君[1,2] 

机构地区：[1]河南医科大学药理教研室 [2]河南医科大学附属卫生学校

出　　处：《河南医学研究》1998年第4期302-303,共2页Henan Medical Research

摘　　要：目的：观察丙咪嗪体外对人血小板胞浆游离钙的影响。方法：用Ｆｕｒａ２－ＡＭ荧光钙离子指示剂在体外测定人血小板胞浆游离钙离子浓度。结果：丙咪嗪明显抑制凝血酶诱导的血小板胞浆游离钙的升高，ＩＣ５０（ｉｎｈｉｂｉｔｏｒｙｃｏｎｃｅｎｔｒａｔｉｏｎｏｆ５０％）为２０．０μｍｏｌ／Ｌ。丙咪嗪对凝血酶诱导的内钙释放亦有明显抑制作用（Ｐ＜０．０１）。Objective: To study the effect of imipramine on intraplatelet free calcium concentration in human platelet,that induced by thrombin.Methods: Using Fura 2 AM fluorescence technique.Results: Imipramine inhibited the rise of [Ca 2+ ] i induce by thrombin in human platelets in vitro significantly.The values of IC 50 was 20.0 μmol/L,respectively.Conclusion: The results suggest that imipramine not only decreases Ca 2+ influx but also inhibits internal Ca 2+ discharge.

关 键 词：丙咪嗪 凝血酶 血小板 钙 依他酸 药理 

分 类 号：R971.43[医药卫生—药品] R965.2[医药卫生—药学]