体外微核试验中吖啶橙荧光染色与Giemsa染色的比较  

A COMPARATIVE STUDY OF ACRIDINE ORANGE FLUORESCENT-AND GIEMSASTAINING METHODS IN THE IN VITRO MICRONUCLEUS TEST.

作　　者：吕晓华[1] 张立实[1] 王瑞淑[1] 

机构地区：[1]华西医科大学营养与食品卫生学教研室,成都610041

出　　处：《现代预防医学》1998年第4期393-394,403,共3页Modern Preventive Medicine

摘　　要：本文在吖啶橙荧光染色(A.O 染色)和常规 Giemsa 染色条件下比较了一组诱变剂丝裂霉素 C(MMC)、5-氟尿嘧啶(5-FU)、长春新碱(VINC)、环磷酰胺(CYP)诱导 L5178Y 和 WTK1细胞的微核试验,结果表明,仅5-FU 和 CYP 在个别时间、剂量下 A.O 染色微核率明显高于 Giem-sa 染色(P<0.05),而绝大多数情况下二者微核率无显著性差异。提示在不具备荧光镜检条件或要求长期保留标本时,Giemsa 染色仍不失为微核染色的理想方法。Acridine Orange(A.O)fluorescent staining method for micronucleus test using L5178Y mouse lymphoma and WTK1 human lymphoblastoid cell lines was systematically compared to the standard Giemsa staining method.The results showed that the micronucleated cell frequencies were not obviously different between A.O- and Giemsa-staining under most conditions.Therefore,it is recommended that Giemsa staining method be avail- able and reliable for micronuclei if fluorescent staining is inadmissibe.

关 键 词：吖啶橙染色 GIEMSA染色 微核试验 L5178Y细胞 

分 类 号：R446.1[医药卫生—诊断学]