瑞芬太尼对家兔缺血脑组织水通道蛋白-4表达的影响  

The Effect of Remifentanil on the Expression of AQP-4 in Rabbits Brain Induced by Cerebral Ischemia-Reperfusion Injury

作　　者：周娜[1] 张蕊[1] 赵兰涛[1] 彭佳宜[1] 李俊杰[1] 韩超[1] 

机构地区：[1]潍坊医学院麻醉学系,山东潍坊261053

出　　处：《潍坊医学院学报》2009年第6期445-447,共3页Acta Academiae Medicinae Weifang

基　　金：潍坊医学院大学生科技创新基金项目资助(课题编号:KX200807)

摘　　要：目的观察瑞芬太尼对家兔缺血再灌注损伤脑组织水通道蛋白4(AQP4)表达的影响。方法健康家兔25只,随机分为假手术组、缺血模型组、瑞芬太尼组。以双侧颈总动脉阻断+控制性低血压法建立家兔全脑缺血损伤模型,再灌注1h,6h,24h末,测定脑组织含水量,观察AQP-4的表达,再灌注24h末,观察脑组织超微结构变化。结果与假手术组比较,模型组家兔脑组织含水量及脑组织内AQP-4表达在1h,6h和24h逐渐升高(P<0.05);与模型组比较,瑞芬太尼组脑组织含水量与AQP-4表达明显降低(P<0.05)。结论瑞芬太尼可能通过降低脑组织内AQP-4表达,减轻脑水肿,保护脑缺血损伤。Objective To study the protective and resuscitative effects of remifentanil on the expression of AQP-4 in rabbits brain. Methods Twenty-five healthy rabbits were randomly divided into three groups ： the sham operation group,the cerebral ischemia group and the remifentanil group. The cerebral isehemia-reperfusion injury model was established by blocking bilateral carotid artery plus the method of controlled hypotension. The moisture capacity of the brain tissue and the expression of AQP-4 was observed at the end of 1 h,6h and 24h after reperfusion. The change of ul- trastructural organization was observed at the end of 24h after reperfusion. Results Compared with sham operation group,the moisture capacity of the brain tissue and the expression of AQP-4 in the cerebral ischemia group were increased gradually after 1 h,6h and 24h（P 〈0.05 ） ;Compared with the cerebral ischemia group, the brain water content and AQP-4 expression in the remifentanil group were obviously lower（ P 〈 0. 05 ）. Conclusion Remifentanil may have cerebral protective effect through downregulating AQP-4 expression in cerebral ischemie area and reducing brain edema.

关 键 词：脑缺血 水通道蛋白-4 瑞芬太尼 超微结构 

分 类 号：R961[医药卫生—药理学]