反转录聚合酶链反应检测自身免疫疾病和正常人外周血淋巴细胞C-myc癌基因的表达  

C-myc ONCOGENE EXPRESSION IN PERIPHERAL BLOOD LYMPHOCYTES FROM NORMAL HUMAN AND PATIENTS WITH AUTOIMMUNE DISEASE BY RT-PCR

作　　者：白建文[1] 邰秀珍[1] 孟丽 高瑞英[1] 

机构地区：[1]内蒙古医学院附属医院

出　　处：《内蒙古医学院学报》1998年第4期248-250,共3页Acta Academiae Medicinae Neimongol

摘　　要：目的：探讨癌基因Ｃ－ｍｙｃ在自身免疫病患者和正常人外周血淋巴细胞上的表达情况。方法：分离正常人及系统性红斑狼疮（ＳＬＥ）、类风湿性关节炎（ＲＡ）患者外周血淋巴细胞，采用异硫氰酸胍一步法提取ＲＮＡ，利用反转录聚合酶链反应（ＲＴ－ＰＣＲ）技术，比较正常人及自身免疫病患者外周血淋巴细胞Ｃ－ｍｙｃｍＲＮＡ表达程度。结果：ＳＬＥ患者外周血淋巴细胞Ｃ－ｍｙｃｍＲＮＡ表达较正常人和ＲＡ患者高，ＲＡ和正常人之间没有差异。ＨＬ－６０细胞系Ｃ－ｍｙｃｍＲＮＡ表达最强，ＧＢＣ胃癌细胞株较正常人为高。结论：提示Ｃ－ｍｙｃｍＲＮＡ在肿瘤细胞株及ＳＬＥ患者外周血淋巴细胞上有较高的表达，认为自身免疫病原癌基因的表达在其发病机理中具有重要的作用。Objective: To study the expression of c-myc in peripheral blood lymphocytes (PBL) from normal human and patients with autoimmune disease. Methods: The lymphocytes from patients with systemic lupus erythematosus(SLE),rheumatoid arthritis(RA) and normal human were separated and total RNA was extracted from lymphocytes by guanidinium isothiocyanate procedure. Using the method of reverse transcriptase polymerase chain reaction(RT-PCR), the level of c-myc mRNA was investigated between normal and autoimmune PBL. Results: PBL from patients with SLE expressed significantly more c-myc mRNA than did controls and RAs. No differences were observed between control subjects and patients with RA. Expression of c-myc mRNA of HL-60 cell line is the highest and GBC cell line is higher than normal. Conclusion:The results indicated that malignant cell line and PBL from SLE have been shown to express an increased level of c-myc mRNA. It is suggested that the expression of oncogene in autoimmune disease may play an important role in the pathogenesis of the disease.

关 键 词：聚合酶链反应 C-MYC基因 反转录 SLE RA 

分 类 号：R593[医药卫生—内科学] R371.23[医药卫生—临床医学]