抗内耳组织的特异性抗体与淋巴细胞免疫示踪研究  

A Labelled Immunological Study of Special Antibodies against Inner Ear Antigens and Lymphocytes

作　　者：谭长强[1,2,3] 曹银成[1,2,3] 刘桦[1,2,3] 童素琴[1,2,3] 何学勤[1,2,3] 

机构地区：[1]南京铁道医学院附属医院耳鼻咽喉科 [2]南京铁道医学院药理学教研室 [3]南京铁道医学院生理学教研室

出　　处：《南京铁道医学院学报》1998年第4期219-221,共3页Journal of Nanjing Railway Medical College

基　　金：国家自然科学基金

摘　　要：目的：了解针对内耳组织的特异性抗体和淋巴细胞到达内耳的途径、部位及其对内耳组织和生理功能的影响。方法：采用荧光标记抗体和同位素标记淋巴细胞，观察被动免疫动物内耳不同部位组织中荧光反应和同位素含量的变化，同时测定听觉功能。结果：特异性抗体（ＩｇＧ）首先到达血管纹部位，内耳听觉功能障碍发生在内耳组织中抗体含量较多时。特异性淋巴细胞则首先出现在内淋巴囊局部，内耳听觉功能损伤亦以ＣＰＭ达峰值时最为明显。结论：针对内耳组织抗原的特异性抗体和淋巴细胞可经过体循环到达内耳局部，抗体主要经血管纹部位造成内耳病变，而特异性淋巴细胞则主要通过内淋巴囊局部造成内耳免疫炎症性病理变化。Objective:The experiment was designed to inquire into the way of the special antibodies against inner ear antigens and lymphocytes to inner ear and their position,and to study their effects on histology and physiological function of the inner ear.Methods:The antibodies marked by fluorescence and T lymphocytes marked by[KG-1〗 3H were used for transmit immunity, then the fluorescence action and the quantity of[KG-1〗 3H in different part of inner ear were observed, and hearing function measured. Results: The special antibodies firstly arrived at striae vascularis, and deafness happened when the fluorescent action was strong. The special lymphocytes appeared in endolymphatic sac first, and the hearing damage arose when the CPM was almost at peak. Conclusions: The special antibodies against inner ear antigens and lymphocytes could get to inner ear through blood circulation system.The antibodies caused immue inflammation in inner ear mainly through striae vascularis whereas the special lymphocytes caused it mainly through endolymphatic sac.

关 键 词：感音神经性聋 免疫示踪 自身免疫病 

分 类 号：R764.430.3[医药卫生—耳鼻咽喉科]