抗CD_3McAb诱导外周血单个核细胞活化增殖的观察  

Observation on the Effects of Anti-CD 3McAb on Activating Human Peripheral Blood Mononuclear Cells

作　　者：许靖霞[1] 蔡仙德[1] 王立新[1] 谭剑萍[1] 

机构地区：[1]南京铁道医学院微生物学教研室

出　　处：《南京铁道医学院学报》1998年第4期231-234,共4页Journal of Nanjing Railway Medical College

基　　金：铁道部科技基金

摘　　要：目的：观察抗ＣＤ３ＭｃＡｂ诱导人外周血单个核细胞（ＨＰＢＭＣ）活化增殖过程中的影响因素及抗ＣＤ３ＭｃＡｂ激活的杀伤细胞（ＣＤ３ＡＫ细胞）免疫学特性的变化。方法：采用ＭＴＴ法和苔盼蓝染色法、ＩＬ－２依赖细胞株（ＣＴＬＬ细胞）增殖实验、ＴＮＦ－α（双抗体夹心ＥＬＩＳＡ法）试剂盒以及间接免疫荧光法分别检测ＣＤ３ＡＫ细胞的增殖活性、内源性ＩＬ－２、ＴＮＦ－α的产生及ＣＤ３、ＣＤ４、ＣＤ８、ＩＬ－２Ｒ等的表达。结果：抗ＣＤ３ＭｃＡｂ对ＨＰＢＭＣ有很强的丝裂原作用，该作用与其诱导浓度、时间及外源性ＩＬ－２的协同作用密切相关。ＣＤ３ＡＫ细胞可产生内源性ＩＬ－２、ＴＮＦ－α，ＩＬ－２Ｒ表达及ＣＤ８＋细胞百分率显著增多。结论：ＣＤ３ＡＫ细胞具有很强的增殖能力，但需要外源性ＩＬ－２的协同作用，增殖细胞以ＣＤ８＋细胞为主。Objective:The experiment was designed to observe the effects of anti-CD 3McAb on the activating and proliferating ability of resting human peripheral blood mononuclear cells (HPBMC) in vitro .Methods:The activating effects of anti-CD 3McAb,the production of endogenous inteleukin-2(IL-2) and the tumor necrosis factors α(TNF-α),and the expression of CD 3,CD 4,CD 8 and IL-2R were obtained by using MTT method,trypan blue test,indirect immunofluorescence method and enzyme-linked immunosorbent assay(ELISA) method.Results:Anti-CD 3McAb was a good mitogenesis,depending on its concentration,time and exogenous IL-2.CD 3AK cells produce both IL-2 and TNF-α.They greatly increase IL-2R expression.There were predominently CD 8-positive cells.Conclusion: Anti-CD 3McAb has a potent mitogenesis that activates T lymphocytes with the presence of synergistic action of exogenous IL-2.

关 键 词：抗CD3McAb HPBMC IL-2 活化增殖 TNF-Α 

分 类 号：R392.12[医药卫生—免疫学]