反相高效液相色谱法测定大鼠血浆中替尼达普钠  

Determination of Tenidap Sodium in Plasma of Rats by Reverse Phase-High Performance Liquid Chromatography

作　　者：许茜[1] 滕杭 贡小清[1] 余书勤[2] 刘歧山[3] 张运生[3] 

机构地区：[1]南京铁道医学院预防医学系 [2]中国药科大学药理学研究室 [3]南京铁道医学院医学科学研究所

出　　处：《南京铁道医学院学报》1998年第4期222-224,共3页Journal of Nanjing Railway Medical College

基　　金：国家科委"九五攻关"

摘　　要：目的：采用反相高效液相色谱法（ＲＰ－ＨＰＬＣ）测定大鼠血浆中替尼达普钠的含量，为其动物药代动力学研究提供方法学基础。方法：分析柱为４．０ｍｍ×２００ｍｍ的ＨｙｐｅｒｓｉｌＢＤＳ，Ｃ１８，柱径１０μｍ，流动相０．２３‰ＫＨ２ＰＯ４∶乙腈＝７０∶３０，含０．１％三乙胺，以Ｈ３ＰＯ４调节ｐＨ至７．６。流速１．０ｍｌ·ｍｉｎ－１，柱温３０℃，紫外检测波长为２３２ｎｍ，进样量１０μｌ。结果和结论：血浆中内源性杂质不干扰替尼达普钠和内标物的测定，相对回收率大于９４％，日内变异系数小于５％，日间变异系数小于１０％，血浆中最低检测浓度为０．１０ｍｇ·Ｌ１。该方法简便可靠。Objective∶A reverse phase-high performance liquid chromatographic(RP-HPLC) method for determination of tenidap sodium in plasma of rats is presented.Methods∶Sample was prepared for analysis by RP-HPLC.The analytical column was a hypersil BDS C 18 column (10μm,4.0mm×200mm),themobile phase was a 70∶30 mixture of 0.23‰ KH 2PO 4-acetonitrile containing 0.1% triethylamine and the pH was adjusted to 7.6,the flow rate was 1.0ml·min -1 and wave length for determination was 232nm.Results and Conclusion∶No interference by endogenous impurity was found.The linear range of the assay was 0.10～50.0mg·L 1 ,the measurable lowest limit of tenidap sodium was 0.10mg·L 1 ,and the recovery rate of the method was higher than 94%.The variation coefficience was less than 5% for within-day and less than 10% for between-day ,respectively.

关 键 词：高效液相色谱法 替尼达普钠 血浆 大鼠 

分 类 号：R971.1[医药卫生—药品]