铜缺乏状态下铜摄入量及铜锌比值对大鼠脂质过氧化的影响  

Effect of copper intake and copper-zinc ratio on rat lipid peroxidation in copper deficiency

作　　者：段链[1] 程义斌[1] 金银龙[1] 

机构地区：[1]中国疾病预防控制中心环境与健康相关产品安全所,北京100020

出　　处：《卫生研究》2010年第1期25-28,共4页Journal of Hygiene Research

基　　金：国际铜业协会(ICA)资助项目(H-AS-07-02)

摘　　要：目的在铜缺乏状态下,控制铜的摄入水平,探讨血中铜浓度及铜锌比值(Cu/Zn)与脂质过氧化各相关指标之间的关系,研究微量元素铜对大鼠脂质过氧化的影响。方法在铜缺乏状态下测定血中铜含量及血中超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、过氧化氢酶(CAT)活性水平及丙二醛(MDA)含量,进行铜对脂质过氧化影响的相关性研究。结果铜摄入不足时给予不同剂量葡萄糖酸铜(Cu-G),各剂量组大鼠血中铜浓度呈现无规律波动,而Cu/Zn比值随着铜摄入量的增加呈递升趋势,结果有统计学意义(P<0.01)。在铜缺乏状态下,大鼠血中SOD活性水平低于正常水平,随着铜摄入量的升高,即血中铜锌比值的升高,大鼠血中SOD活性水平呈现上升趋势。血中MDA含量随着铜摄入量的增加(即铜锌比值的升高)会有所下降,并保持在某一较低水平。血中铜锌比值的下降可能导致大鼠血中CAT活性水平下降,但铜锌比值与CAT活性的相关关系呈非线性相关。铜摄入量对大鼠血中GSH-Px无明显影响。结论在铜缺乏状态下,大鼠血中SOD活性下降,MDA含量上升;随着血中铜锌比值的升高,SOD活性呈上升趋势,同时血中MDA含量下降到较低水平。而铜摄入不足会使血中CAT的活性水平有所升高,对GSH-Px活性水平无明显影响。Objective To study the relations among the copper concentration,the copper-zinc ratio （Cu/Zn） and the biomarkers of lipid peroxidation by controlling the level of copper intake in copper deficiency,to analyze the effect of trace element copper on the rat lipid peroxidation.Methods Determine the content of copper in blood and the activity of superoxide dismutase （SOD）,glutathione peroxidase （GSH-Px） and catalase （CAT） and the content of malondialdehyde （MDA）,carrying out the relevant research.Results The rats were treated with copper gluconate （Cu-G） in different doses in the case of insufficient copper intake,the copper concentration in the blood of each group rats presented irregular fluctuate,while the copper-zinc ratio （Cu/Zn） showed a ascending trend following the increase of copper intake,and the results have statistical significance （P〈0.01）.In the status of copper deficiency,the SOD activity is lower than the normal level,and then presented a rise trend with the increased of copper intake that is,the ratio of copper-zinc （Cu/Zn）.The content of MDA is higher than the normal level,and the content of MDA decreased with the increased of copper intake and the Cu/Zn ratio,and maintained at a relatively low level.The decreased of Cu/Zn ratio could induce the decrease of the CAT activity in the rat blood,while there were no linear correlation be found between them.And no significant effect on the GSH-Px activity in the rat blood in inadequate of copper intake.Conclusion In copper deficiency,the SOD activity in the rat blood would be lower than the normal level and the content of MDA would increase in rat blood,while with the increase of Cu/Zn ratio,the SOD activity increased gradually,and the content of MDA dropped to a lowed level.While the insufficient intake of copper could induce a increase of the CAT activity in blood and no effect on GSH-Px.

关 键 词：铜 铜缺乏 脂质过氧化 超氧化物歧化酶 谷胱甘肽过氧化物酶 过氧化氢酶 丙二醛 

分 类 号：Q58[生物学—生物化学] Q591