氟对体外培养大鼠大脑皮层星形胶质细胞的毒性作用  

Toxic effects of fluoride on rat cerebral cortex astrocytes in vitro

作　　者：李红霞[1] 黄厚今[1] 徐园园[1] 高艳玲[1] 刘振环[1] 

机构地区：[1]遵义医学院预防医学教研室,遵义563003

出　　处：《卫生研究》2010年第1期86-88,共3页Journal of Hygiene Research

基　　金：贵州省省长专项基金(黔省专合字No.2009-125);贵州省自然科学基金(黔科合J字No.2007-2126);遵义医学院博士启动基金

摘　　要：目的探讨NaF对体外培养大鼠大脑皮层星形胶质细胞细胞周期和琥珀酸脱氢酶(SDH)、5′-核苷酸酶(5′-NT)、酸性磷酸酶(ACP)活性的影响。方法体外分离纯化培养获得纯度达98%以上的大鼠大脑皮层星形胶质细胞(特异性抗GFAP免疫化学鉴定),分别用浓度为1、2和5mmol/LNaF对星形胶质细胞染毒12、24、48和72h,FCM检测细胞周期构成比,紫外比色法检测SDH、5′-NT、ACP活性。结果FCM分析细胞周期显示:1～5mmol/L剂量范围内,NaF能够以剂量依赖方式诱导星形胶质细胞周期由S期阻滞向G2/M期阻滞转变,随着时间延长变化更明显,超出这一范围则主要为subG1期细胞。紫外比色法检测结果表明,NaF抑制SDH、ACP活性,2mmol/L范围内对5′-NT活性影响无显著性(P>0.1),增加剂量5′-NT失活;SDH、ACP活性与subG1DNA期细胞相对含量显著负相关(SDH:r=-0.84148,P<0.05;ACP:r=-0.90416,P<0.01)。结论NaF可诱导星形胶质细胞细胞周期阻滞及凋亡,并可抑制其SDH、5′-NT、ACP活性。Objective To study astrocytes cellular cycle and the activities of SDH,5＇-NT and ACP in rat cerebral cortex astrocytes by NaF in vitro.Methods Astrocytes of rat cerebral cortex were purified and cultured in vitro to obtain the highly purified cells （identified by immunochemistry of anti-GFAP）.The constituent ratio of cell cycle was analyzed by FCM after the cells were cultured with different levels of NaF for different time span.Activities of SDH,5＇-NT and ACP were tested by UV colorimetry.Results Cell cycle arrest transited from S phase to G2/M phase and subG1 cells increased in 1-5mmol/L in the astrocyte cycle treated with different levels of NaF for different time span by using FCM.There was inverse correlationship between the relative amount of the subG 1 DNA and activities of SDH and ACP respectively （SDH：r=-0.84148,P〈0.05;ACP：r=-0.90416,P〈0.01） that were inhibited by NaF and 5mmol/L NaF can inactivate 5＇-NT in astrocytes.Conclusion NaF can induce cell cycle arrest from S to G2/M and inhibit activities of 5＇-NT,SDH and ACP in astrocytes.

关 键 词：氟 星形胶质细胞 细胞周期 酶活性 

分 类 号：R599.9[医药卫生—内科学] Q58[医药卫生—临床医学]