红汁乳菇固体培养条件响应面法的初步研究  

Primary Study on Solid Cultural Condition of Lactarius hatsudake by Response Surface Method

作　　者：李瑞婧[1] 曹文涛[1] 王志[1] 陈志龙[1] 高龙超[1] 王佳[1] 陈靖[1] 

机构地区：[1]贵州大学化学与化工学院,贵州贵阳550003

出　　处：《贵州农业科学》2010年第3期72-75,共4页Guizhou Agricultural Sciences

基　　金：贵州省科学技术基金项目"野生红汁乳菇深层培养及多糖分离提取工艺的研究"[黔科合J字(2008)2088];贵大本科生SRT项目"红汁乳菇土壤化学营养即最适培养条件的研究"[贵大SRT字(2009)121]

摘　　要：在单因素试验的基础上,采用响应面分析法对影响红汁乳菇固体培养的主要因素(pH、温度和VB含量)进行优化,建立影响因素与响应值(菌落直径)之间的函数关系。根据回归方程寻优得出,最佳工艺条件为pH6.5、温度23.5℃、VB含量为56.9mg/L。红汁乳菇菌落的最大直径为78.5mm。结果表明:响应面法对红汁乳菇固体培养条件优化合理,可为工业化生产提供一定理论依据。Three main factors of pH, temperature and VB content affecting solid culture of Lactarius hatsudake were optimized by response surface method based on the single factor test and the relationship between factors and colonial diameter was established. The optimum technological condition for solid culture of Lactarius hatsudake was pH 6.5, 23.5℃ and 56.9 mg/L VB content, and the maximum colonial diameter of Lactarius hatsudake was up to 78. 5mm under the optimum technological condition. The optimization results could provide a theoretical basis for industrialization production.

关 键 词：红汁乳菇 培养基优化 响应曲面法 

分 类 号：S646.9[农业科学—蔬菜学]