犬蛔虫成虫抗原的促B细胞有丝分裂的活性  

MITOGENIC ACTIVITY OF TOXOCARA CANIS ADULT WORM ANTIGEN ON B CELL

作　　者：姜洪杰[1] 牛春[1] 诸欣平[1] 张帆[1] 黄松[1] 

机构地区：[1]首都医科大学寄生虫学教研室

出　　处：《中国寄生虫病防治杂志》1998年第4期291-294,共4页Chinese Journal of Parasitic Disease Control

摘　　要：在体内用正常小鼠脾及人体外周血淋巴细胞就犬弓蛔虫抗原（ＴｃＡｇ）对淋巴细胞的作用进行了研究。成虫的ＴｃＡｇ在浓度为１～１２５μｇ／ｍｌ时能刺激小鼠脾细胞增殖。ＴｃＡｇ的应答细胞为Ｂ细胞，因为经抗免疫球蛋白抗体和补体处理或尼龙棉柱分离后，应答消失。此应答不是由脂多糖（ＬＰＳ）的污染引起的，因为ＴｃＡｇ能刺激作为ＬＰＳ的低应答者的Ｃ３Ｈ／ＨｅＪ脾细胞。ＴｃＡｇ不但刺激增殖反应，而且刺激多克隆ＩｇＧ和ＩｇＥ产生。ＴｃＡｇ还刺激巨噬细胞产生白细胞介素－１并刺激人的Ｂ细胞。上述结果还表明ＴｃＡｇ是一个有效的促Ｂ细胞有丝分裂原，其活性与感染犬弓蛔虫宿主的免疫功能的改变有关。The effect of Toxocara(T) canis antigen (TcAg) on lymphocytes was studied in vitro using normal murine spleen cells and human peripheral blood lymphocytes. TcAg prepared from adult worms at concentration of 1-125 μg/ml was able to stimulate murine spleen cells to proliferate. The responders for TcAg were B cells, because the response was depleted by the treatment of spleen cells with anti immunoglobulin (Ig) antibody as well as complement or separation on a nylon wool column. This response was not due to the contamination of lipopolysaccharide(LPS), because C3H/HeJ spleen cells which are low responders to LPS could be stimulated by TcAg. Not only the proliferative response but also the production of polyclonal IgE and IgG was stimulated by TcAg. TcAg could also stimulate macrophages to produce interleukin 1 and stimulate human B cells. These results suggested that TcAg was a potent B cell mitogen and this activity may be relevant to the alteration of immune functions in hosts infected by T. canis .

关 键 词：犬弓蛔虫 B细胞 巨噬细胞 增殖 

分 类 号：R532.110.3[医药卫生—内科学]