叶酸对初产母猪繁殖性能和宫内发育迟缓仔猪肾脏功能基因表达的影响  

Effects of Folic Acid Supplementation on Reproductive Performance of Gilts and Kidney Functional Gene Expression in Newborn Piglets Suffered Intrauterine Growth Restriction

作　　者：刘静波[1] 姚英[1] 余冰[1] 陈代文[1] 

机构地区：[1]四川农业大学动物营养研究所,雅安625014

出　　处：《动物营养学报》2010年第2期278-284,共7页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：教育部创新团队猪抗病营养分子机制研究项目(IRTO555-5)

摘　　要：本试验旨在研究叶酸对初产母猪繁殖性能和宫内发育迟缓(IUGR)仔猪肾脏DNA甲基转移酶-1(DN-MT-1)、p53基因、Bcl-2、Bax和胰岛素样生长因子-1(IGF-1)基因表达的影响。选用24头初产母猪,配种后将母猪随机分为对照组(C,1.3mg/kg叶酸)和试验组(F,30.0mg/kg叶酸),每组12个重复,每个重复1头母猪,记录母猪分娩24h内的繁殖性能指标以及21日龄断奶时的仔猪断奶重;采集母猪妊娠60d时血清,以及正常出生体重(NBW)和IUGR新生仔猪血清和肾脏,用放射免疫分析法测定血清叶酸浓度;用荧光定量PCR(RT-PCR)方法研究DNMT-1、p53基因、Bcl-2、Bax和IGF-1在肾脏中的表达差异。结果表明:母体添加30.0mg/kg叶酸极显著提高初产母猪和新生仔猪血清叶酸含量(P<0.01),但对初产母猪繁殖性能无显著影响(P>0.05)。IUGR+C组DNMT-1表达量显著低于NBW组(P<0.05);IUGR+C组p53表达量极显著高于NBW组和IUGR+F组(P<0.01);IUGR+C组Bcl-2表达量极显著低于NBW组和IUGR+F组(P<0.01);IUGR+F组DNMT-1、p53和Bcl-2表达量与NBW组差异不显著(P>0.05);IUGR组Bax表达量显著高于NBW组(P<0.05);IUGR组IGF-1表达量显著低于NBW组(P<0.05);IUGR组和NBW组的Bax和IGF-1表达量组内差异都不显著(P>0.05)。以上结果揭示出:补充叶酸对初产母猪繁殖性能无显著影响,极显著提高初产母猪和新生仔猪血清叶酸含量。通过母体补充叶酸可缓解IUGR对DNMT-1、p53和Bcl-2基因表达的影响。This experiment was aimed to study the effects of maternal folic acid supplementation on reproductive performance of gilts and kidney mRNA expression of DNA methyltransferasel （DNMT-1）, p53, Bax, Bcl-2 and insuline-like growth factor-1 （IGF-1） of newborn piglets suffered intrauterine growth restriction （IUGR）. Twenty-four gilts were randomly allocated to two groups, control group （C, folic acid 1.3 mg/kg） and folic acid supplementation （F, folic acid 30.0 mg/kg） group with 12 replicates each. Gilts＇ reproductive performance within 24 h after parturition and weight of weanling piglets at 21- day-old was recorded. Gilts＇ blood samples at the 60th day of pregnancy, and normal body weight （NBW） and IUGR new born piglets＇ blood samples and kidney weres collected for serum folic acid concentration test by radio immunoassay. Real- time polymerase chain reaction （RT-PCR） was applied to determine gene expression differences of DNMT-1, p53, Bel-2, Bax and IGF-1 in kidney. The results showed as follows： supplementation with 30.0 mg/kg folic acid significantly increased serum folic acid concentration of gilts and piglets （P（0.01）, but there was no significant difference in reproductive per- formance of gilts; real-time PCR indicated that relative mRNA expression of DNMT-1 was lower in IUGR ＋ C group than in NBW groups （P〈0.05）, and transcript expression level of p53 in IUGR ＋ C group was higher than that in NBW groups and IUGR ＋ F group （P〈0.01）, while Bcl-2 in IUGR ＋ C group was lower than that in NBW groups and IUGR ＋ F group （P〈 0.01）, and there was no significant difference in expression of DNMT-1, p53 and Bcl-2 between IUGR ＋ F and NBW groups （P〉0.05） ; the expression abundance of Bax was higher in IUGR groups than in NBW groups and the opposite pattern was noted for IGF-1 （P〈0.05）, no significant differences of kidney Bax and IGF-1 expression was noted within IUGR and NBW groups, respectively （P〉0.05）. Results indicated that suppl

关 键 词：叶酸 初产母猪 繁殖性能 基因表达 宫内发育迟缓 

分 类 号：S828[农业科学—畜牧学] S816.7[农业科学—畜牧兽医]