利用实时定量PCR研究不同蛋白质饲料对嗜淀粉瘤胃杆菌生长参数的影响  

Effects of Different Protein Feeds on Growth Parameters of Ruminobacter amylophilus in the Rumen by Real-time PCR

作　　者：王梦芝[1] 詹爱军[2] 高杨[1] 徐爱秋[1] 喻礼怀[1] 王洪荣[1] 

机构地区：[1]扬州大学动物科学与技术学院,扬州225009 [2]深圳出入境检验检疫局动植物检验检疫技术中心,深圳518010

出　　处：《动物营养学报》2010年第2期327-334,共8页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：国家自然科学基金资助项目(编号:30771567);扬州大学博士启动基金

摘　　要：本试验主要研究不同蛋白质饲料对嗜淀粉瘤胃杆菌(Ruminobacter amylophilus)生长的影响。选用4只装有瘤胃瘘管的徐淮白山羊羯羊,分别饲喂以羽毛粉、玉米蛋白粉、豆粕和鱼粉为蛋白质补充料的4组日粮,进行4×4拉丁方设计的试验,并采用实时定量PCR(RT-PCR)技术对嗜淀粉瘤胃杆菌进行定量分析。结果表明,豆粕组瘤胃氨氮浓度最高,并显著高于其他3组(P<0.05);细菌总数在组间差异不显著(P>0.05);嗜淀粉瘤胃杆菌的密度及其数量在细菌总数中的比例均以豆粕组最高,且组间差异显著(P<0.05)。总之,豆粕饲料适宜于嗜淀粉瘤胃杆菌的生长,RT-PCR技术适用于瘤胃微生物的定量研究。The objective of this paper was to investigate the effects of different protein feeds on growth parameters of Ruminobacter amylophilus in rumen. Four Xuhuai white goats fitted with rumen cannula, were assigned randomly in a 4 × 4 Latin square, and were fed with 4 protein supplementation diets which were plume meal group, corn gluten meal group, soybean meal group, and fish meal group, respectively. The Real-time PCR technique was introduced to determine the quantity of Ruminobacter amylophilus. The results showed that NH3-N concentration was the highest in soybean meal group, and significantly higher than that in the other 3 groups （P 〈0. 05）. No significant difference was found in total amount of bacteria among all groups （P〉0.05） ; while both Ruminobacter amylophilus density and the percentages of Rurninobacter amylophi- lus in all bacteria were the highest in soybean meal group, and there were significant differences among all groups （P〈 0.05）. In conclusion, soybean meal feed was suitable for Ruminobacter amylophilus growth, and real-time PCR technique was a powerful method for ruminal microorganism quantity analysis.

关 键 词：蛋白质饲料 嗜淀粉瘤胃杆菌 生长 实时定量PCR 

分 类 号：S823[农业科学—畜牧学] S816.4[农业科学—畜牧兽医]