云南拟单性木兰组织培养初步研究  

Tissue culture of Parakmeria yunnanensis Hu

作　　者：甘露[1,2] 李凯[1,2] 王小青[1,2] 陈雄庭[1] 

机构地区：[1]中国热带农业科学院,海南海口571101 [2]海南大学,海口570228

出　　处：《广西农业科学》2010年第3期210-212,共3页Guangxi Agricultural Sciences

基　　金：国家科技基础条件平台建设资助项目(2005DKA21000-5-42)

摘　　要：以不同季节的云南拟单性木兰幼嫩茎段为外植体,探讨外植体不同消毒时间、不同激素配比对腋芽萌发和生长以及芽增殖的影响。结果表明,取于6月份的木兰幼嫩茎段不适宜作为外植体进行培养;2.5%NaClO 15 min+0.2%HgCl2 8 min对外植体的消毒效果最好;最适的腋芽诱导培养基为MS+6-BA 0.5 mg/L+2,4-D 0.1 mg/L+KT 0.5mg/L+蔗糖5%+琼脂0.6%,增殖培养基为1/2MS+6-BA 0.5 mg/L+NAA 0.1 mg/L+GA3 0.1 mg/L+蔗糖5%+琼脂0.6%。An experiment was undertaken to study the effects of different sterilization times （5, 8, 10 and 15 min） using 0.2% HgCl2 and hormone combinations on induction and growth and multiplication of axillary buds of Parakmeria yunnanensis Hu by using young shoots sampled in different months （April, June, and September） as explants. The results showed that young shoots sampled in June were unsuitable for tissue culture due to higher browning rate and lowest mortality rate. Sterilizing the shoots in 2.5% NaCl0 solution for 15 min followed by 0.2% HgC12 solution for 8 min gave best results. The medium suitable for induction and multiplication of axillary buds was found to be MS ＋ 6-BA 0.5mg/L ＋ 2,4-D 0.1mg/L ＋ KT 0.5mg/L ＋ sugar 5%＋ agar 0.6% and, I/2MS ＋ 6-BA 0.5 mg/L ＋ NAA 0.1mg/L ＋ GA3 0.1mg/L ＋ sugar 5% ＋agar 0.6%.

关 键 词：云南拟单性木兰 腋芽 诱导 增殖 

分 类 号：S685.150.1[农业科学—观赏园艺]