低钙高氟对大鼠脾组织细胞核因子κB受体活化因子配体mRNA表达的影响  

Effects of low calcium and high fluoride on expressions of receptor activator of nuclear factor-kappa B ligang mRNA in spleens of rats

作　　者：王春红[1] 王秀丽[1] 卢爱平[1] 徐辉[2] 李广生[2] 井玲[2] 

机构地区：[1]吉林大学第二医院血液科,长春130041 [2]吉林大学第二医院医学部地方病研究所病理室,长春130041

出　　处：《中国地方病学杂志》2010年第2期127-129,共3页Chinese Jouranl of Endemiology

基　　金：国家自然科学基金（30271156）

摘　　要：目的探讨低钙高氟对大鼠脾组织细胞核因子KB受体活化因子配体（RANKL）mRNA表达的影响。方法Wistar大鼠40只，体质量（118．9±13．5）g。采用2×2×2析因设计，将大鼠按体质量随机分为4组：对照组（常规饲料）、低钙组（低钙饲料）、高氟组（常规饲料＋高氟饮水100mg／L）和低钙高氟组（低钙饲料＋高氟饮水100mg／L），每组10只。在喂养4和8个月时各处死5只大鼠，取大鼠脾脏，抽提脾组织中的总RNA，采用RT-PCR方法分析RANKL mRNA的表达。结果4个月时对照组、低钙组、高氟组、低钙高氟组RANKL mRNA表达分别为0．13±0．05、0．13±0．03、0．17±0．02、0．27±0．05；8个月时分别为0．11±0．01、0．16±0．02、0．16±0．03、0．36±0．07。析因分析显示。低钙、高氟对RANKL mRNA表达有影响（F值分别为40．224、56．679，P均〈0．05），作用时间对RANKL mRNA表达无影响（F=2．850，P〉0．05）；低钙高氟、低钙与作用时间对RANKL mRNA表达存在交互作用（F值分别为7．247、18．789，P均〈0．05），高氟与作用时间对RANKL mRNA表达无交互作用（F=1．751．P〉0．05）。结论低钙或高氟单独作用或低钙和高氟协同作用促进大鼠脾组织RANKL mRNA表达；低钙促进RANKL mRNA表达与作用时间有关，高氟促进RANKL mRNA表达不受作用时间影响。Objective To study the different expressions of receptor activator of nuclear factor-kappa B ligang（RANKL） mRNA in spleens of rats fed with diet of low calcium and high fluoride. Methods A 2 × 2 × 2 factorial design was used and the factors were calcium, fluoride and action time. In the design, 40 Wistar rats [average body mass（118.9 ± 13.5）g] were divided into four groups randomly by weight： control with normal diet （0.790%, calcium）, low calcium group with low calcium intake（0.063%, calcium）, high fluoride group with normal diet and high fluoride intake（100 mg/L, fluoride） and low calcium and high fluoride group with low calcium and high fluoride intake. After 4 and 8 months, 5 rats of each group were sacrificed and total RNA was extracted from spleen. And the expression levels of RANKL mRNA were determined by reverse transcription polymerase chain reaction （RT-PCR）. Results At time of 4 months, the expression level of RANKL mRNA was 0.13 ± 0.05,0.13 ± 0.03,0.17 ± 0.02,0.27 ± 0.05 and at time of 8 months, it was 0.11 ±.01,0.16 ± 0.02,0.16 ± 0.03,0.36± 0.07 in control group, low calcium group, high fluoride group, low calcium with high fluoricte group, repectively. The factorial design AVONA showed that low calcium and high fluoride had significant effects on RANKL mRNA expression（F = 40.224,56.679, all P 〈 0.05） while action time had not（F = 2.850, P 〉 0.05）. The interactions of low calcium with high fluoride or high fluoride with action time were significant（F = 7.247,18.789, all P 〈 0.05） while the interaction of high fluoride with action time was not（F = 1.751, P 〉 0.05）. Conclusions Low calcium alone or high fluoride alone or low calcium with high fluoride or low calcium with action time can increase the the RANKL mRNA expression level. High fluoride does not affect the RANKL mRNA level as the action time is prolonged.

关 键 词：氟 钙 脾 细胞核因子κB受体活化因子 

分 类 号：R686[医药卫生—骨科学]