硒对氟中毒鸡淋巴细胞亚群及脾淋巴细胞凋亡的影响  

Effect of selenium on apoptosis of spleen cells, T-cell subset of fluorosis chicken

作　　者：黄蔚[1] 关丽萍[1] 徐世文[1] 崔雅莉[1] 李术[1] 

机构地区：[1]东北农业大学动物医学学院,哈尔滨150030

出　　处：《中国地方病学杂志》2010年第2期142-145,共4页Chinese Jouranl of Endemiology

摘　　要：目的观察硒对慢性氟中毒鸡血液、脾脏CD4^＋、CD8^＋淋巴细胞亚群以及脾淋巴细胞凋亡的影响，并探讨其作用机制。方法将8日龄海蓝褐雏鸡180只按体质量随机分为3组：对照组、氟中毒组、硒拮抗组，每组60只，分别饲以含氟195、1000、1000mg／kg、含硒0.08、0．08、4．00mg／kg的全价日粮。于30、60、90d用流式细胞术检测外周血和脾脏中CD4^＋、CD8^＋淋巴细胞水平，TUNEL法检测脾淋巴细胞凋亡情况。结果与对照组比较，30、60、90d氟中毒组外周血CD4^＋淋巴细胞水平降低[（35．36±4．27）％vs（24．29±2．96）％、（47．65±5．42）％vs（41．62±3．96）％、（49．58±3．98）％vs（42．35±6．03）％，P〈0．05或〈0．01]，CD4^＋／CD8^＋比值也明显降低[（1．701±0．145）％vs（1．393±0．163）％、（2．712±0．345）％vs（1．781±0．201）％、（2．438±0．356）％vs（1．973±0．229）％，P〈0．05或〈0．01]；与氟中毒组比较，30、60、90d硒拮抗组外周血中CD4^＋淋巴细胞水平升高[（29．40±3．38）％、（45．40±6．01）％、（46．85±5．25）％，P〈0．05或〈0．01]，60、90d时CD4^＋／CD8^＋比值明显回升[（2．004±0．314）％、（2．211±0．229）％，P均〈0．01]。与对照组比较，30、60、90d氟中毒组脾脏中CD4^＋淋巴细胞水平降低[（47．33±5．35）％vs（41．91±4．83）％、（49．28±5．24）％vs（41．26±4．56）％、（34．31±4．15）％vs（29．33±2．89）％，P均〈0．01]，CD4^＋／CD8^＋比值也明显降低[（1．927±0．244）％vs（1．525±0．265）％、（1．847±0．224）％vs（1．640±0．198）％、（1．265±0．174）％vs（0．878±0．092）％，P〈0．05或〈0．01]；与氟中毒组比较，60、90d硒拮抗组脾脏中CD4^＋淋巴细胞水平升高[（44．87±5，43）％、（32．62±3．37）％，P均〈0．05]，而30、60、90d时CD4^＋／CD8^＋比值明显回升Objective To study the effect of selenium on peripheral and splentic T-cell subset, apoptosis of spleen cells in fluorosis chicken and its mechanism. Methods One hundred and eighty 8-day Hailanhe chicks were randomly divided into 3 groups （each 60） ： ①control group： 195 mg/kg fluoride and 0.08 mg/kg of selenium; ②fluorine group： 1000 mg/kg fluoride and 0.08 mg/kg of selenium;③selenium antagonism group： 1000 mg/kg fluoride and 4.00 mg/kg of selenium. On 30^th, 60^th, 90^th day, peripheral and splentic CD4^＋, CD8^＋ T-cell subset analyses underwent flow cytometry and apoptosis of spleen cells were detected by TUNEL for study subjects. Results Compared with control group, the CD4^＋ T-cell subset of peripheral in fluorine group was decreased obviously in 30,60,90 days[ （35.36 ± 4.27）% vs （24.29 ± 2.96）%, （47.65 ± 5.42）% vs （41.62 ± 3.96）%, （49.58 ± 3.98）% vs （42.35 ± 6.03）%, P〈 0.05 or 〈 0.01 ], CD4^＋/CD8^＋ ratio also was decreased obviously[ （1.701 ± 0.145）% vs （1.393 ± 0.163）%,（2.712 ± 0.345）% vs （1.781 ± 0.201）%, （2.438 ± 0.356）% vs （1.973 ± 0.229）%, P〈 0.05 or 〈 0.01 ]. Compared with fluorine group, the CD4^＋ T-cell subset of peripheral in selenium antagonism group [ （29.40 ± 3.38）%, （45.40 ± 6.01）%, （46.85 ± 5.25）%, P 〈 0.05 or 〈 0.01 ] was increased obviously in 30,60,90days, CD4^＋/CD8^＋ ratio in 60,90 days[ （2.004 ± 0.314）%, （2.211 ± 0.229）%, all P 〈 0.01] also was increased obviously. Compared with control group, the CD4^＋ T-cell subset of spleen cells in fluorine group was decreased obviously in 30,60,90 days[（47.33 ± 5.35）% vs （41.91 ± 4.83）%, （49.28 ± 5.24）% vs （41.26 ± 4.56）%, （34.31 ± 4.15）% vs （29.33 ± 2.89）%, all P 〈 0.01 ], CD4^＋/CD8^＋ ratio also was decreased obviously[ （1.927 ± 0.244）% vs （1.525 ± 0.265）%, （1.847 ± 0.224）% vs （1.640 ± 0.198）%, （1.265±0.174）% vs （0.878 ± 0.092）%, P 〈 0.05 or 〈 0.01 ]

关 键 词：硒 氟 细胞凋亡 免疫 T淋巴细胞亚群 

分 类 号：R686[医药卫生—骨科学]