乙酰胆碱对海马CA1区痛反应神经元电活动的影响  

Effect of Acetylcholine on Electric Discharge of Pain-related Neurons in Hippocampal CA1 Area

作　　者：肖宇[1] 杨晓芳[2] 徐满英[3] 

机构地区：[1]齐齐哈尔医学院生理学教研室,161006 [2]复旦大学脑科学研究院,上海200032 [3]哈尔滨医科大学生理学教研室,150086

出　　处：《医学研究杂志》2010年第3期43-45,共3页Journal of Medical Research

基　　金：国家自然科学基金资助项目(30240058)

摘　　要：目的研究侧脑室注射乙酰胆碱(ACh)对大鼠海马CA1区痛反应神经元电活动的影响。方法以电脉冲刺激右侧坐骨神经作为伤害性刺激,用玻璃微电极细胞外记录神经元放电。结果脑室注射ACh(20μg/10μl)可使痛兴奋神经元(PEN)放电频率的净增值减少,潜伏期延长;使痛抑制神经元(PIN)放电频率的净增值增加,完全抑制时程明显缩短。结论 ACh能够减弱海马CA1区内的痛反应神经元对伤害性刺激的反应,表现为镇痛效应。Objective To study the effect of intracerebroventricular （icv） injectionon acetylcholine （ACh） on the elecric activities of pain - related neurons in the hippocampal CAl area of rat. Methods Trains of the electric impulses applied to the right sciatic nerve were used as noxious stimuli. The discharges of neurons were led out by extracellular recording method with glass microelectrodes. Results Icy injection of ACh （20μg/10μl） caused an decrease of the evoked discharge frequency of pain -excited neurons （PEN） and a prolongation latency as well as an increase of the evoked discharge of pain -inhibited neurons （PIN） and a shortening of inhibitory duration. Conclusion The response of pain - related neurons in hippocampal CA1 area to the noxious stimulation is weakened by icv injection of ACh, exhibiting analgesic effect.

关 键 词：乙酰胆碱 海马CA1区 痛兴奋神经元 痛抑制神经元 电活动 

分 类 号：R338.3[医药卫生—人体生理学]