磷脂酶A_2、白细胞介素-1β、金属蛋白酶组织抑制物-1在创伤性脑损伤中的表达研究  

Study of the Expression of the Phospholipase A_2,Interleukin-1β and Tissue Inhibitor of Matrix Metalloproteinase-1 Following Traumatic Brain Injury

作　　者：李廷富[1] 万立华[2] 张薇[3] 傅登俊 

机构地区：[1]成都市第三人民医院检验科,646000 [2]重庆医科大学法医教研室,400016 [3]泸州医学院附属医院康复科,646000 [4]成都市双流县第一人民医科院检验科,610200

出　　处：《医学研究杂志》2010年第3期58-61,共4页Journal of Medical Research

基　　金：四川省科技厅基金项目[川科技(2006)6]

摘　　要：目的研究胞质型磷脂酶A2（cPLA2）、白细胞介素-1β（IL-1β）、金属蛋白酶组织抑制物-1（TIMP-1）在创伤性脑损伤中的表达以及作用。方法以自由落体撞击伤大鼠模型为对象，用RT—PCR方法检测脑组织中cPLA2、IL-1β、TIMP-1的mRNA表达变化。结果cPLA2的mRNA在伤后2h表达升高，7天达到高峰，以后下降，但直到14天时仍高于正常对照组水平。IL-1β在伤后1h表达明显升高，5～8h达到高峰，在伤后72h时降至正常对照组水平。TIMP-1在伤后2h开始升高，48～72h达到高峰，到14天时基本接近正常对照组水平。结论cPLA2、IL-1β和Timp-1在脑损伤后表达增加提示cPLA2、IL-1β和TIMP-1参与了脑损伤过程并可能在其中发挥了重要作用。Objective To investigate the expression of the cytusolic phospholipase A2 （ c - PLA2 ）, interleukin - 1 beta（ IL - 1β）, tissue inhibitor of matrix metalloproteinase - 1 （TMP - 1 ） in rat brain after injury. Methods The model of traumatic brain injury originally described by Feeney was employed. The mRNA was analyzed by RT - PCR . Results The mRNA of cytosolie phospholipase A2 was increased at 2 hour and its peak was at 7 day. At 14 day, the level of cytosolic phosphalipase A2 mRNA was still in high level as compared to the con- trol group, l.ikely, the enhancement expression of interleukin- 1β was seen at the 1 hour and the peak time was from 5 to 8 hour . At 72 hour, it decreased to normal level. The expression of inhibitor of matrix metalloproteinase - 1 was increased at the 2 hour, and the highest expression level was seen during 48 to72 hour . It came down to normal level at 14 day after injury. Conelusion The augment of the expression of the cytosolie phospholipase A2 , interleukin - 1β and tissue inhibitor of matrix metalloproteinase - 1 following traumatie brain injury suggested that they participated in the pathogenesis of the traumatic brain injury,and may played roles in this pathophysiological proeess.

关 键 词：磷脂酶A2 白介素-1Β 基质金属蛋白酶抑制剂-1脑损伤 

分 类 号：R961[医药卫生—药理学]