取食B型烟粉虱前侵染的烟草对B型烟粉虱和烟蚜体内保护酶和消化酶活性的影响  

Effects of feeding on tobacco plants preinfested by Bemisia tabaci (Homoptera:Aleyrodidae) B-biotype on activities of protective enzymes and digestive enzymes in B.tabaci and Myzus persicae (Homoptera:Aphididae)

作　　者：毕明娟[1] 薛明[1] 李庆亮[1] 王洪涛[1] 刘爱红[1] 

机构地区：[1]山东农业大学植物保护学院昆虫学系,山东泰安271810

出　　处：《昆虫学报》2010年第2期139-146,共8页Acta Entomologica Sinica

基　　金：国家自然科学基金项目(30671379;30971906);山东省烟草专卖局资助项目(KN72)

摘　　要：前期研究发现,重要的外来入侵害虫B型烟粉虱Bemisia tabaci在烟草植株上对烟蚜Myzus persicae具有明显的种间竞争优势。为明确两种害虫的生理适应性差异对其种间竞争的影响,用生化分析法研究了两种成虫取食B型烟粉虱为害的烟草植株后其体内主要保护酶和消化酶活性的影响。结果表明:B型烟粉虱取食其前侵染的烟草植株6,12,24,48和72h后,其体内超氧化物歧化酶(SOD)和多酚氧化酶(PPO)的活性均显著增加(P<0.05),过氧化物酶(POD)活性在取食12和24h后变化不明显,但在取食6,48和72h后也显著增加(P<0.05),B型烟粉虱体内的这3种酶活性的最高值较对照最高分别增加达1.62,2.71和2.57倍。烟蚜取食B型烟粉虱前侵染的烟草植株在多数取食时间段内,其体内PPO和SOD的活性仅略有增加,但在取食12h后SOD的活性受到明显的抑制。POD活性除在取食24h后显著高于对照外,在取食其他时间后均受到显著的抑制(P<0.05)。B型烟粉虱取食其前侵染的烟草植株不同时间后,其体内蛋白酶和淀粉酶活性均显著增加,最大值分别是对照的1.54倍和1.33倍;烟蚜取食烟草植株6h后,其体内蛋白酶和淀粉酶活性与对照相比没有明显差异(P<0.05),但随着取食时间的延长,这两种酶活性被显著抑制,其最低值分别为对照的0.39和0.72倍。结果提示:与烟蚜相比,B型烟粉虱取食经B型烟粉虱前侵染的烟草后其体内保护酶和消化酶的活性提高是其具有较高适应性的原因之一。Previous studies indicated that Bemisia tabaci B biotype, an important invasive pest, had the advantage in interspecific competition with Myzus persicae on tobacco. To explore the effect of physiological adaptability of the two insects on their interspecific competition, the changes of activities of main protective enzymes and digestive enzymes in B. tabaci and M. persicae feeding on tobacco preinfested by B. tabaci were studied using biochemical analysis. The results showed that the activities of PPO and SOD in B. tabaci significantly increased after feeding on the preinfested tobacco plants for 6, 12, 24, 48 and 72 h （P 〈 0.05 ）, and the POD activity in B. tabaci also significantly increased after feeding on the preinfested tobacco plants for 6, 48 and 72 h, respectively （P 〈 0.05）. The maximum activities of SOD, PPO and POD in B. tabaci on the preinfested tobacco plants were 1.62, 2.71 and 2.57 times higher than those in the control, respectively. In contrast, the activities of PPO and SOD in M. persicae increased a little in most time-span after feeding on the preinfested tobacco plants, while the SOD activity was obviously suppressed after feeding on the preinfested tobacco plants for 12 h （P 〈 0.05 ）. POD activity in M. persicae was significantly inhibited except after feeding on the preinfested tobacco plants for 24 h （P 〈 0.05 ）. Activities of protease and amylase in B. tabaci significantly increased in all time-span after feeding on the preinfested tobacco plants, and the maximum activities were 1.54 and 1.33 times that in the control, respectively. Activities of protease and amylase in M. persicae did not change after feeding on the preinfested tobacco plants for 6 h （ P 〈 0.05 ） ; however, the activities were inhibited with the extending of feeding time, and the lowest activities in M. persicae feeding on the preinfested tobacco plants were 0.39 and 0.72 times that in the control, respectively. The results suggest that the increase of the activities of protective and diges

关 键 词：B型烟粉虱 烟蚜 前侵染 保护酶 消化酶 生理适应性 

分 类 号：Q965.9[生物学—昆虫学]