幼虫信息素中三种酯类对中华蜜蜂和意大利蜜蜂工蜂哺育和封盖行为以及蜂王发育影响  

Effects of three aliphatic esters of brood pheromone on worker feeding and capping behavior and queen development of Apis cerana cerana and A.mellifera ligustica

作　　者：曾云峰[1] 曾志将[1] 颜伟玉[1] 吴小波[1] 

机构地区：[1]江西农业大学蜜蜂研究所,南昌330045

出　　处：《昆虫学报》2010年第2期154-159,共6页Acta Entomologica Sinica

基　　金：国家自然科学基金项目(30760035);国家蜂产业技术体系科学家岗位项目(nycytx-43-kxj15)

摘　　要：为研究蜜蜂幼虫信息素中3种酯类成分(甲基棕榈酸酯、乙基棕榈酸酯和乙基油酸酯)对中华蜜蜂Apiscerana cerana和意大利蜜蜂Apismellifera ligustica工蜂哺育行为、封盖行为以及蜂王发育影响,在人造蜂蜡王台中加入1%和0.1%(w/w)的3种酯类作为实验组,以不添加酯类(0%)的为对照组,移入1日龄工蜂幼虫,测定王台接受率、单个王台中幼虫和王浆重量;另将分别添加1%和0.1%(w/w)3种酯类的石蜡假幼虫放入工蜂巢房中,同样设对照组,测定假幼虫的封盖率;在新鲜王浆中以1%和0.1%(w/w)分别加入3种酯类作为实验组,以不添加酯类(0%)的作为对照组,再分别在1日龄、2日龄和3日龄幼虫王台中加入0.01mL含有酯类的蜂王浆,并测定蜂王初生重和卵巢管数量。结果表明:0.1%甲基棕榈酸酯可以显著提高中蜂和意蜂幼虫重量;意蜂的甲基棕榈酸酯和乙基油酸酯两个实验组(1.0%,0.1%)假幼虫封盖率都极显著高于对照组;乙基油酸酯两个实验组(1.0%,0.1%)都显著降低了中蜂和意蜂蜂王初生重和卵巢管数量。这说明不同蜜蜂幼虫信息素具有不同的生物学效应。To study the effects of brood pheromone esters （methyl palmitate, ethyl palmitate and ethyl oleate） on feeding and capping behavior of workers and development of the queens of Apis cerana cerana and A. mellifera ligustica, 1-day-old worker larvae were transferred to the artificial queen cells made of bee wax that were mixed with three aliphatic esters at concentrations of 1% and 0.1% （w/w）, and then the acceptance of queen cells, weight of each larva, and weight of royal jelly in each queen cell were tested. Dummy larvae made of paraffin mixed with three aliphatic esters at the concentrations of 1% and 0.1% （w/w）, respectively, were introduced into the empty cells, and the worker acceptance of cells was tested. After dripping royal jelly mixed with three aliphatic esters at the concentrations of 1% and 0.1% （w/w）, respectively, into the queen cells when the larvae was 1-, 2- and 3-day-old, the emergence weight and the quantity of ovarioles of queen were tested. The results showed that （1） Methyl palmitate （0. 1% ） significantly improved the weight of larvae in each queen cell in both A. cerana cerana and A. mellifera ligustica. （2） Methyl palmitate （ 1%, 0.1% ） and ethyl oleate （ 1%, 0.1% ） significantly increased the capping rate of the dummy larvae in A. mellifera ligustica. （ 3 ） Ethyl oleate （ 1%, 0.1% ） significantly depressed weight and ovarioles of the queen in both A. cerana cerana and A. mellifera ligustica. These results indicate that different brood pheromones of honeybee have different biological effects.

关 键 词：中华蜜蜂 意大利蜜蜂 幼虫信息素 哺育行为 封盖行为 蜂王发育 

分 类 号：Q966[生物学—昆虫学]