中华蜜蜂工蜂视叶的胚后发育  

Postembryonic development of the optic lobes in the Chinese honeybee,Apis cerana cerana (Hymenoptera:Apidae)

作　　者：李兆英[1,2] 奚耕思[1] 

机构地区：[1]陕西师范大学生命科学学院,西安710062 [2]陕西教育学院生命科学系,西安710061

出　　处：《昆虫学报》2010年第2期160-166,共7页Acta Entomologica Sinica

基　　金：陕西省自然科学研究计划项目(2005C125)

摘　　要：为了研究蜜蜂视叶的胚后发育模式,本研究通过形态解剖、免疫组织化学技术,对中华蜜蜂Apis cerana cerana工蜂视叶的胚后发育过程进行了系统的比较研究。结果表明:中华蜜蜂的视叶起源自幼虫早期脑内部的两个视原基。外部视原基经过不对称的细胞分裂产生神经节母细胞,随后这些细胞经过快速的对称分裂,复制自身并生成视髓层神经细胞;外部视原基的极少数细胞分裂产生视神经节层神经节母细胞,到蛹发育中期,随着视神经进入的刺激,神经节层神经细胞才开始快速增殖,并最终形成了视神经节层的所有结构。内部视原基的分裂方式同外部视原基相同,最终生成视叶的视小叶部分。本研究结果提示中华蜜蜂的视叶起源自两个视原基,大多数神经细胞在前蛹期产生,视神经的进入刺激了视神经节层的发育。The postembryonic development of optic lobes in Apis cerana cerana was comparatively studied by using immunohistochemical method （5-bromo-2-deoxyuridine, BrdU immunostaining） and in situ cell death detection. The results showed that the medulla and lamina were generated by distinct populations of neuroblasts in the outer optic anlage （OOA）, and the neuroblasts divided asymmetrically to generate ganglion mother cells. Ganglion mother cells later divided symmetrically to generate immature neurons. Generation of the medulla cortex started with the onset of the final larval instar. Generation of the lamina cortex was initiated with the arrival of retinal afferents at the optic lobes. The lobula neurons were generated by neuroblasts in a second structure, the inner optic anlage （IOA）. The results suggest that the optic lobe was generated from two optic anlages, the proliferation of neurons showed a peak at prepupa, and generation of the lamina cortex was initiated with the arrival of retinal afferents at the optic lobes.

关 键 词：中华蜜蜂 工蜂 视叶 胚后发育 免疫组织化学 

分 类 号：Q962[生物学—昆虫学]