资源昆虫角倍蚜遗传多样性及遗传分化的AFLP分析  

Genetic diversity of the resource insect Schlechtendalia chinensis (Homoptera:Pemphigidae) populations in China based on AFLP analysis

作　　者：吕姝媛[1] 段立柱[1] 马恩波[2] 任竹梅[1] 

机构地区：[1]山西大学生命科学与技术学院,太原030006 [2]山西大学应用生物学研究所,太原030006

出　　处：《昆虫学报》2010年第2期202-208,共7页Acta Entomologica Sinica

基　　金：国家自然科学基金项目(30670361);山西省自然科学基金项目(2007011078)

摘　　要：本研究采用AFLP分子标记技术对资源昆虫角倍蚜Schlechtendalia chinensis6个种群共102个个体的样本进行遗传多样性分析,探讨角倍蚜主要分布区不同种群之间的遗传分化及其变异程度,为合理利用和保护该经济昆虫提供分子方面的证据。结果表明:经过摸索实验筛选出的4对选择性扩增引物共扩增条带126条,多态性条带比例为100%,种群多态性位点比例介于23.81%～66.67%之间;Nei’s基因多样性指数介于0.0942～0.1980之间;Shannon’s多样性数介于0.1381～0.3027之间;AMOVA分析显示57.99%的变异来源于种群内,42.41%的变异来源于种群间(P<0.01);总群体的Fst值为0.4242;NJ聚类树显示角倍蚜6个种群共形成两个大的聚类簇,阳雀、丹寨和汉中种群聚为一支,安县、竹山和龙胜3个种群聚为另一支。总体上,角倍蚜种内的遗传多样性较低,而种群间的遗传分化较大,遗传距离与地理距离之间没有显著的相关关系(P>0.05)。The genetic diversity and genetic structure of six populations （totally 102 individuals） of the resource insect Schlechtendalia chinensis in China were examined using amplified fragment length polymorphism （AFLP） method in order to provide molecular evidence for proper utilization and protection of this economic insect. Four pairs of selective primers were used to amplify the total DNA of S. chinensis samples with AFLP method. The results indicated that the primers yielded a total of 126 scorable loci, of which 100% was polymorphic. The percentage of polymorphic loci in different populations changed from 23.81% to 66.67%, Nei＇ s gene diversity index （H） from 0. 0942 to 0. 1980, and Shannon＇ s index （I） from 0. 1381 to 0. 3027. AMOVA analysis showed that 57.99% genetic variation came from intra- populations, 42.41% genetic variation from inter-populations, and the overall Fst was 0.4242. NJ cluster analysis showed that the six populations are grouped into two major clades （A and B ） , each consisting of three populations. Clade A includes three populations （Yangque, Danzhai and Hanzhong）, and clade B includes the other three populations （ Anxian, Zhushan and Longsheng）. The results suggest that the genetic diversity of S. chinensis populations is low, but the genetic differentiation among populations is high. The genetic diversity among populations is not related to the geographic distance （P 〉0.05）.

关 键 词：角倍蚜 AFLP技术 遗传多样性 遗传结构 聚类关系 

分 类 号：Q961[生物学—昆虫学]