家庭内红色毛癣菌病患者间菌株差异性分析  

Variability analysis of Trichophyton rubrum isolates in members of the same family

作　　者：何晓丹[1] 冉玉平[1] 代亚玲[1] 青春[1] 张浩[1] 向耘[1] 

机构地区：[1]四川大学华西医院皮肤性病科,成都610041

出　　处：《中国真菌学杂志》2010年第1期5-8,21,共5页Chinese Journal of Mycology

基　　金：教育部高等学校博士学科点专项基金(20050610058)

摘　　要：目的用PCR技术比较分离自同一家庭红色毛癣菌病患者的菌株差异性,分析家庭内多发的红色毛癣菌病的致病菌株是家内相互感染,还是家外感染。方法以家庭内多发的皮肤癣菌病患者为研究对象,分离致病菌株并以传统方法鉴定菌种。再分别用随机扩增多态性DNA(RAPD)和巢式PCR特异扩增红色毛癣菌的串联重复亚元件(TRSS:TRS-1/TRS-2)产生的指纹图谱分析种内株间有无差异性。结果纳入实验的16株菌分离自8个家庭,用形态学等方法及种特异引物均鉴定为红色毛癣菌。RAPD显示4个家庭内的菌株间有差异性,TRS-1区PCR指纹图谱显示5个家庭内菌株有株间差异,TRS-2区能鉴定出2个家庭内菌株间有差异。综合各方法共区分出6个家庭内的菌株间有带型差异。结论该研究提示家庭内多发红色毛癣菌病从家外途径感染率高于家内感染。TRS-1区PCR指纹图谱对红色毛癣菌的菌株区分度高于RAPD,更适于红色毛癣菌株间分型。结合多种分子分型方法可最大限度发现不同菌株间的差异。Objective To compare the cariability of the Trichophyton rubrum strains isolated from different members by PCR in the same family and to determine the dermatophytosis were transmitted within the family or infected from outside. Methods Scales scraped from patients with dermatophytosis in the same fainily were inoculated on SDA. T. rubrum was identified by physiological and morphological characteristics. If strains from different patients were found to belong to the same species, RAPD and specific amplification of tandem repeat sub-elements （ TRSS ） in strain typing were performed and the products were compared. Results All the strains isolated from 16 patients in 8 families （2 patients per family） were identified as T. rubrum by morphological methods. In 4 families, distinction was identified by RAPD with 4 arbitrary primers, in 5 families the isolates from each family were found to be different in the types of fingerprints produced by amplifying the TRS-1 region specifically. Fingerprints of TRS - 2 amplified from isolates differred in 2 families . In all differences were found in strains from 6 families . Conclusions Dermatophytosis in one family is more frequently infected from outside than inside. Fingerprints produced by specifically amplifying TRS-1 are more effective in distinguishing strains of T. rubrum spp than RAPD, and TRS-1 is more suitable for strain typing in T. rubrum. RAPD combimed with TRSS may distinguish T. rubrum strains to maximum.

关 键 词：红色毛癣菌 分子分型 家庭 

分 类 号：R379.9[医药卫生—病原生物学]