表面活性剂增敏分光光度法测定L-色氨酸  

Determination of L-tryptophan by surfactant-sensitized spectrophotometry

作　　者：郑民[1,2] 徐素琴[1] 朱霞石[1] 

机构地区：[1]扬州大学化学化工学院,江苏扬州225002 [2]扬州商务高等职业学校,江苏扬州225127

出　　处：《扬州大学学报（自然科学版）》2010年第1期34-37,共4页Journal of Yangzhou University：Natural Science Edition

基　　金：国家自然科学基金资助项目(20875082)

摘　　要：采用分光光度法测定Trp—NO2^-体系的吸收光谱行为，加入表面活性剂Triton X-114胶束后，溶液的吸光度增大，体系得到增敏，由此建立一种快速简便测定L-色氨酸含量的方法．结果表明：色氨酸量在0．2～34．0mg·L^-1范围与其吸光度呈线性关系，其直线回归方程为A＝0．777ρ-0．0119，相关系数r=0．994，检出限为0．054mg·L^-1，该方法具有灵敏度高、操作简捷的优点．在优化参数条件下对粮食等多种样品中的L－色氨酸进行分析，结果令人满意．The absorption spectral behavior of the system Trp-NO2 is studied in Triton X-114 medium. Absorbance of the system increases with the addition of the surfactant micelle Triton X-114, thereby enhances the sensitivity of the system. Based on this, a novel surfactant-sensitized spectrophotometry method for the determination of L-tryptophan has been developed. The results show that under the optimal conditions, the linear range of calibration curve for the determination of L- tryptophan is 0.2-34.0 mg·L^-1 , and the detection limit is 0. 054 mg·L^-1. It has been applied to the determination of L-tryptophan in food samples with satisfactory results.

关 键 词：L-色氨酸 表面活性剂 增敏 分光光度法 

分 类 号：O657.32[理学—分析化学] O629.71[理学—化学]