重组甘精胰岛素对糖尿病大鼠模型代谢影响的实验研究  

The Experimental Study of Recombinant Insulin Glargine in Diabetic Rat Model of Metabolic Effects

作　　者：曾敏莉[1] 周远大[2] 

机构地区：[1]重庆医科大学附属儿童医院,重庆400014 [2]重庆医科大学附属第一医院,重庆400016

出　　处：《儿科药学杂志》2010年第2期10-13,共4页Journal of Pediatric Pharmacy

摘　　要：目的:研究长效胰岛素重组甘精胰岛素对糖尿病大鼠代谢紊乱的调节作用。方法:将糖尿病模型大鼠40只随机分为5组,每组8只,雌雄各半。分为正常对照组、模型对照组、重组胰岛素注射液大、中、小剂量组和阳性对照组。主要观察精神、进食、体重等指标,测定试验大鼠血糖、糖化血红蛋白定量,血清中TG、TC、TP、Alb含量及血清中SOD、XOD酶活性、MDA含量。结果:重组甘精胰岛素注射液治疗组及阳性对照组大鼠平均体重增加,治疗组及阳性对照组大鼠血糖浓度较模型组血糖浓度均有降低(P<0.05);血清甘油三酯的含量降低(P<0.05),总胆固醇降低(P<0.05),增加血清总蛋白水平、血清白蛋白水平,升高血清SOD的活性(P<0.05),降低XOD的活性(P<0.05),降低MDA的含量(P<0.05);降低糖尿病大鼠血尿素氮的含量(P<0.05);降低糖尿病大鼠糖化血红蛋白的含量(P<0.05)。结论:重组甘精胰岛素对糖尿病大鼠的代谢紊乱有调节作用。Objective： To study the effect of long-acting recombinant insulin, Glargine insulin, on metabolic disorders in diabetic rats in vitro. Methods： Forty diabetic rats were randomly divided into five groups with eight in each and same numbers of males and females （a model control group, insulin injection of large, medium and low-dose groups, and a positive control group）. Their spirit, feeding, urine output, weight and so on were observed, and tests to determine the quantity of their glucose, glycosylated hemoglobin and their TG, TC, TP, Alb and SOD, XOD activity and MDA content in serum were measured. Results： The recombinant Glargine insulin injection treatment group and positive control group got their average weight gained, the treatment group and the positive control group were lower in blood glucose concentration levels compared with the model group （P 〈 0.05）. Serum triglyceride levels were also lower （P 〈 0.05）, the total cholesterol became lower （ P 〈 0.05 ）, total protein and serum albumin level increased, serum SOD activity increased （ P 〈 0.05 ）, XOD activity decreased （P 〈 0.05 ）, MDA content significantly was lower （ P 〈 0.05 ）. Diabetic rats had lower levels of blood urea nitrogen （P 〈 0. 05 ） and glycosylated hemoglobin （ P 〈 0.05 ）. Conclusions ： Recombinant insulin Gglargine in diabetic rats have a moderating role in the metabolic disorder.

关 键 词：重组甘精胰岛素 代谢紊乱 糖尿病 

分 类 号：R587.1[医药卫生—内分泌]