Micropropagation of Pistacia vera 'Kerman'  

阿月浑子的组织培养和快速繁殖(英文)

作　　者：于菲[1] 苏淑钗[2] 沈漫[1] 姬谦龙[3] 冷平生[4] 

机构地区：[1]北京农学院植物科学技术系,北京102206 [2]北京林业大学林学院,北京100083 [3]北京农学院组培中心,北京102206 [4]北京农学院园林系,北京102206

出　　处：《Agricultural Science & Technology》2010年第1期107-108,121,共3页农业科学与技术（英文版）

基　　金：Supported by Beijing Natural Science Foundation (6072005);Funding Project for Academic Human Resources Development in Institutions of Higher Learning Under the Jurisdiction of Beijing Municipality(PXM2009-014207-076874)~~

摘　　要：[Objective]The aim was to commercialize micropropagation of pistachio and provide good foundations for varieties improvement. [Method]The seeds and stem-segments were used as explants in the tissue culture of Pistacia vera ＇Kerman＇,tissue culture condition and medium and transplant matrix were researched for micropropagation of pistachio,[Result]Mediums of 1/2DKW and 1/2DKW＋3.00 mg/L of 6-BA ＋0.05 mg/L of NAA were suitable medium for seed germination and axillary bud induction of pistachio; Proliferation coefficient was 3.6 on the medium of 1/2DKW ＋4.00 mg/L of 6-BA ＋ 0.05 mg/L of IBA; Rooting rate was up to 75% on the medium of 1/2DKW ＋ 5.00 mg/L of IBA ＋ 1.00 mg/L of NAA; The suitable transplantation matrix was 3/4 sand ＋ 1/4 vermiculite,and survival rate of plant with root was above 79%. [Conclusion]The pistachio （Kerman） tissue culture technology system was established.以阿月浑子良种‘科尔曼’的种子和枝条作为外植体进行组织培养。结果表明:1/2DKW和1/2DKW+6-BA3.0mg/L+NAA0.05mg/L作为种子萌发和茎段分化的培养基;1/2DKW+6-BA4.0mg/L+IBA0.05mg/L为增殖培养基,增殖系数可达3.6;1/2DKW+IBA5mg/L+NAA1mg/L为生根培养基,生根率75%以上,移栽在河砂与蛭石(3∶1)混合基质中,成活率达79%以上。

关 键 词：Pistachio （Pistacia vera L.） Tissue culture TRANSPLANT 

分 类 号：S664.9[农业科学—果树学]