反相高效液相色谱法测定血浆中利福喷丁的浓度  

Determination of rifapentine in human plasma by HPLC with UV detection

作　　者：夏志高[1] 

机构地区：[1]广州市第一人民医院药剂科,510180

出　　处：《广州医药》2010年第2期59-61,共3页Guangzhou Medical Journal

摘　　要：目的建立反相高效液相色谱法测定人血浆中利福喷丁的浓度。方法以Kromasil 1850mm×4．6mm，5μm）为分析柱，甲醇：0．02mol／L，KH2P02（磷酸调pH5．2，65：35v／v）为流动相，流速为1．0ml／min，检测波长为335nm，利福平为内标，测定血浆中利福喷丁的浓度。结果在0．316～20．2μg/ml浓度范围内，利福喷丁和内标峰的面积比值与浓度呈良好的线性关系（r=0．9991）。利福喷丁高、中、低3个浓度的平均回收率分别为：103．80％，98．53％，102．65％；日内、日间RSD均小于10％。结论该方法简便、快速准确、重现性好，可用于药代动力学以及生物等效性研究。Objective To establish a RP-HPLC method for the determination of rifapentine in human plasma. Meth- ods Using Kiomasal （ C18, 4. 6 mm× 150 mm, 5 μm） analytical column, the mobile phase consisted of methanol ： 0. 02 mol/ L KH2PO4 （adjusting pH =5.2 by otrhophosphoric acid, 65： 35） at the flow rate 1.0 ml/min. Serum concentration of rifapentine and internal standard （rifampicin） were determined at UV 335 nm. Results The linear ranges of rifapentine were 0. 316 20. 2 μg/ml （r =0. 9991 ） . The average recoveries for three concentration of rifapentine were 103.80% , 98.53% , 102. 65% , respectively. The RSD of within-day and between-day was less than 10%. Conclusion The method is convenient, fast, repro- ducible and suitable for pharmacxokinetic study of rifapentine.

关 键 词：利福喷丁 反相高效液相色谱法(HPLC) 血药浓度 测定 

分 类 号：R96[医药卫生—药理学]