小鼠HMGN2干扰质粒psilence-cmv-4.1-HMGN2构建及体内干扰效果鉴定  

Construction and identification of mouse shRNA psilencer-cmv-4.1-HMGN2

作　　者：韩琴[1,2] 邓璐霞[1] 赵静[1] 吴桂霞[1] 曹玥[1] 范波[1] 李夏[1] 陈善泽[1] 高祥[1] 黄宁[1] 

机构地区：[1]四川大学华西医学中心感染与免疫实验室,四川成都610083 [2]成都医学院公共卫生实验中心,四川成都610041

出　　处：《四川生理科学杂志》2010年第1期5-8,共4页Sichuan Journal of Physiological Sciences

基　　金：国家自然科学基金(30671963);CMB基金(98-683)

摘　　要：目的:构建小鼠HMGN2干扰质粒psilence-cmv-4.1-HMGN2,以进一步明确HMGN2的生物学作用。方法:将HMGN2-shRNA片段退火后与小鼠psilence-cmv-4.1链接,饲养孕小鼠,尾静脉注射法导入shRNA表达质粒,提取8.5d胚胎RNA,用RT-PCR检测胎HMGN2的表达量进行鉴定。结果:测序结果显示psilence-cmv-4.1-HMGN2干扰质粒构建成功,RT-PCR结果显示HMGN2在8.5d胎儿表达较高广泛表达,且Psilencer-cmv-4.1-HMGN2尾静脉注射后有明显的抑制效果。结论:成功构建对8.5d胎鼠HMGN2基因具有显著干扰效率的psilencer-4.1-HMGN2干扰质粒,为进一步研究HMGN2基因的功能打下了基础。Objective： To construct highly efficient mouse shRNA psilencer-c mv-4. 1-HMGN2, and the transcription mechanism of HMGN2. Methods. shRNA psileneer-c mv-4. 1-HMGN2 was constructed and was transmitted into the pregnant ICR mice with tail venous injection. At 8. 5 days in gestation, embryo RNA was extracted and identified the expression of HMGN2 by RT-PCIL Resuits：The shRNA psilenee-emv-4. 1-HMGN2 was constructed successfully and the expression of HMGN2 was higher at 8. 5 days in gestation embryo, and significantly downregulated by shRNA psilencer-c mv-4. 1-HMGN2. Conclusion. The psilenee-c mv-4. 1- HMGN2 was constructed successfully and the interference efficiency was 70%.

关 键 词：HMGN2 Psilencer-cmv-4.1-HMGN2 

分 类 号：R346[医药卫生—基础医学]