切换波长法同时测定芫花中芫花素与芫花酯甲含量  

Determination of Genkwain and Yuanhuacine in Buds of Daphne Genkwa by HPLC

作　　者：董倩[1] 邓仕任[1] 陈晓霞[1] 张振秋[1] 夏林波[1] 

机构地区：[1]辽宁中医药大学药学院,辽宁大连116600

出　　处：《辽宁中医药大学学报》2010年第4期69-70,共2页Journal of Liaoning University of Traditional Chinese Medicine

基　　金：国家科技支撑计划子课题(2006BA109B06-07-1)

摘　　要：目的:建立芫花药材中芫花素与芫花酯甲含量的测定方法。方法:采用高效液相色谱切换波长法同时测定芫花素与芫花酯甲的含量。色谱条件为Diamonsil C18色谱柱(250mm×4.6mm,5μm);流动相:甲醇-水(84:16);检测波长:前14min为338nm,14min后为232nm;柱温:25℃;流速:1mL/min。结果:此方法线性良好,芫花素与芫花酯甲的平均加样回收率分别为97.7%,97.0%,RSD分别为2.28%,1.64%(n=5)。结论:该方法简便、可靠、重复性好,可用于芫花药材中芫花素与芫花酯甲的同时检测。Objective： To establish the analytical method for genkwain and yuanhuacine in the buds of Daphne genkwa by means of HPLC. Method： Genkwain and yuanhuaeine were determined by HPLC simultaneously with changing ultraviolet-visible wavelength. ODS-C18（250mm × 4.6mm,5 μ m） column was used.A mixture of methanol-water（84：16） was used as the mobile phase with a flow rate of 1.0mL/min. UV detection wavelength of genkwain was set at 338nm and yuanhuaeine at 232nm. The contents were calculated by external-standard method. Results： The average recoveries were 97.7% and 97.0% and RSD were 2.28% and 1.64%（n=5）. Conclusion： The method can be used to determine genkwain and yuanhuacine in the buds of Daphne genkwa simultaneously, and it appears to be convenient, reliable and repeatable.

关 键 词：芫花 HPLC 芜花素 芫花酯甲 切换波长 

分 类 号：R284.2[医药卫生—中药学]