抗癌扶正药方多糖的含量测定  

Determination of the Polysaccharides of Ait-tumor and Enhancing Immunity

作　　者：许桂玲[1] 皮文霞[1] 叶丽红[1] 

机构地区：[1]南京中医药大学,江苏南京210046

出　　处：《辽宁中医药大学学报》2010年第4期83-84,共2页Journal of Liaoning University of Traditional Chinese Medicine

基　　金：江苏省中医药局课题(HLO7O81)

摘　　要：目的:为了增强抗癌扶正方多糖的抗肿瘤活性,建立多糖的含量测定方法。方法:在传统水提醇沉的基础上采取了多次无水乙醇、丙酮、乙醚纯化工艺,应用0.2%蒽酮一硫酸法测定多糖含量。结果:经过初步的纯化工艺得出抗癌扶正方的多糖含量为24.9%。结论:该方法操作简便,稳定性较好,可以用于测定抗癌扶正方多糖的含量。Objective： In order to enhance the anti-tumor activity of polysaccharide compound, to establish the method for the content determination of the polysaccharides. Method： On the basis of the traditional water extraction and alcohol precipitation, it adopted a preliminary purification process of anhydrous ethanol, ethyl ether, acetone. It applies of the anthrone sulfuric acid method of determination of polysaecharide. Result： After a preliminary purification procedure of polysaecharides, the compound gets the compound content of 24.9%. Conclusion： The method is simple and stable. It can be used for determination of the polysaccharides.

关 键 词：抗癌扶正方 多糖 含量测定 

分 类 号：R284.1[医药卫生—中药学]