评估SELDI-TOF-MS技术检测肾移植术后病人尿液的能力  

Evaluation of the Capability of SELDI-TOF-MS Detecting Protein from Urine After Renal Transplantation

作　　者：陈雅斌[1] 赵猛[2] 兰小鹏[1,2] 

机构地区：[1]福建医科大学福州总医院临床医学院 [2]福州总医院全军医学检验研究所,福建福州350025

出　　处：《生物技术通讯》2010年第2期236-239,共4页Letters in Biotechnology

基　　金：南京军区医学科学技术研究"十一五"计划(06Z47)

摘　　要：目的:研究表面增强激光解吸电离飞行时间质谱(SELDI-TOF-MS)技术检测肾移植术后病人尿液中蛋白质的能力。方法:根据尿液标本中蛋白质浓度进行稀释处理,采用SELDI-TOF-MS技术,用3种不同芯片(NP20、CM10、IMAC30)与蛋白质浓度的不同组合分别检测肾移植术后病人的尿液。结果:采用NP20/蛋白质未稀释、CM10/蛋白质浓度约0.1g/L、IMAC30/蛋白质浓度约2g/L时,质谱图中蛋白质峰的个数和丰度达到最佳;单一芯片中NP20捕获质荷比为5000～20000的蛋白质能力最佳,而CM10和IMAC30在质荷比为2000～5000时有较大的捕获能力;3种芯片中CM10具有最大的捕获蛋白质的能力。结论:3种芯片检测尿液中蛋白质的能力不同;为了更好地发现疾病特异性标志物,最好多种芯片同时检测,且在各种芯片得到最佳质谱图时的浓度进行检测。Objective：To evaluate the capability of surface-enhanced laser desorption /ionization time-of-flight mass spectrometry（SELDI-TOF-MS） detecting protein from urine after renal transplantation.Methods：Urine samples were diluted to considerable concentration that determined by its total protein concentration,and were detected using NP20,CM10 and IMAC30 chips by SELDI-TOF-MS.Results：The best concentrations of capture protein outlooking more peaks and more strong intensity in CM10,IMAC30 and NP20 were 0.1 g /L,2 g /L and no diluted,respectivly.The best capture proteins capability on NP20 was at the range from 5000 to 20 000 M /Z,but on CM10 and IMAC,was from 2000 to 5000 M /Z.In peaks and intensity,CM10 performed best.Conclusion：In order to screen the disease-specific protein,urine samples should be detected by varied chips at the same time and at their best concentrations.

关 键 词：表面增强激光解吸电离飞行时间质谱 尿液 检测 肾移植 

分 类 号：Q503[生物学—生物化学] R446[医药卫生—诊断学]