动物线粒体DNA分析技术及其应用  

Analysis Techniques of Animal Mitochondrial DNA and Their Application

作　　者：闫华超[1] 

机构地区：[1]聊城大学生命科学学院,山东聊城252059

出　　处：《生物技术通讯》2010年第2期280-283,294,共5页Letters in Biotechnology

基　　金：山东省教育厅科技计划(J08LE02)

摘　　要：结合动物线粒体基因组的最新研究进展,对目前动物线粒体基因序列的分析技术进行了概述,分析了应用较广的限制性片段长度多态性(RFLP)、序列特异性寡核苷酸分析(SSO)、DNA芯片、单链构象多态性(SSCP)、变性梯度凝胶电泳(DGGE)和竞争性寡核苷酸引物延伸(COP)等技术的原理、方法和适用范围,并对它们在线粒体基因的遗传、起源和进化等热点问题中的应用进行了阐述。In this paper, the analysis techniques of animal mitochondrial DNA were summarized from the lastst researching outcomes. Some analysis techniques, such as restriction fragment length polymorphism（RFLP）, sequencespecific oligonucleotide（SSO）, DNA chip, single strand conformational polymorphism（SSCP）, denaturing gradient gel electrophoresis（DGGE） and competitive oligonucleotide priming（COP） were introduced, and their principles, methods and applicabilities were emphasized. This paper gave important information for the application of these techniques on genetics and evolution of mitochondrial genes.

关 键 词：线粒体DNA 分析技术 应用 

分 类 号：Q343[生物学—遗传学] Q523