鼠肝高尔基体双向凝胶电泳的建立  

Development of Two-dimensional Gel Electrophoresis for Rat Liver Golgi Apparatus

作　　者：李艳青[1] 易永芬[1] 何婷婷[1] 肖钟[1] 

机构地区：[1]重庆医科大学病理教研室分子医学与肿瘤研究中心,重庆400016

出　　处：《中国生物制品学杂志》2010年第3期310-312,共3页Chinese Journal of Biologicals

基　　金：国家自然科学基金(30672431);教育部博士点基金(20060631006)

摘　　要：目的分离纯化鼠肝高尔基体,初步建立一套相对完善、分辨率和重复性均较高的高尔基体双向凝胶电泳技术。方法应用蔗糖密度梯度超离心法分离纯化高尔基体,通过电镜观察和标志酶分析鉴定其形态及纯度,双向凝胶电泳分离高尔基体蛋白质,用PDQuest8.0软件分析电泳图谱。结果电镜观察显示大部分为高尔基体,纯化高尔基体标志酶TPP酶比活力提高了120倍,得到了分辨率和重复性均较好的双向凝胶电泳图谱。结论已成功建立了鼠肝高尔基体双向凝胶电泳技术。Objective To isolate and purify rat liver Golgi apparatus and develop a relatively perfect two-dimensional gel electrophoresis（2-DE）with high resolution and reproducibility.Methods Golgi apparatus was isolated and purified by sucrose density gradient centrifugation,and identified for purity by electron microscopy and analysis of marker enzyme.The protein of Golgi apparatus was separated by 2-DE,and the 2-DE profile was analyzed by PDQuest8.0 software.Results Electron microscopy showed that the majorities of isolates were Golgi apparatus.The specific activity of marker enzyme TPP of purified Golgi apparatus increased by 120 folds,and the 2-DE profile with high resolution and reproducibility were obtained.Conclusion The 2-DE technique for rat liver Golgi apparatus was successfully developed.

关 键 词：鼠肝 高尔基体 双向凝胶电泳 

分 类 号：Q246[生物学—细胞生物学] R392.33[医药卫生—免疫学]