益气养阴、消癥通络中药对大鼠肾小球系膜细胞分泌细胞外基质的影响  

Effect of herb of supplement qi and nourish yin eliminating mass and dredge nels and collaterals on the mesangial cells secreting extracellular matrix

作　　者：张江华[1] 陈志强[1] 孙玉凤[2] 赵雯红[1] 王月华[3] 王会青[1] 李玉梅[1] 

机构地区：[1]河北医科大学中西医结合研究所,河北石家庄050011 [2]河北医科大学附属第二医院消化科 [3]河北医科大学中医院肾内科

出　　处：《中国老年学杂志》2010年第5期620-622,共3页Chinese Journal of Gerontology

基　　金：河北省自然科学基金资助项目(No.C2008001074);河北省卫生厅资助项目(No.08165)

摘　　要：目的探讨益气养阴、消癥通络中药药物血清对大鼠系膜细胞细胞外基质(ECM)不同成分的抑制作用。方法以体外培养大鼠肾小球系膜细胞及血清药理学方法为基础,采用酶联免疫吸附试验检测ECM中纤维连接蛋白(FN)、层黏连蛋白(LN)、Ⅳ型胶原(ColIV)的含量。结果FN、ColⅣ的含量均增加,而益气养阴、消癥通络中药对其具有明显抑制作用。结论益气养阴、消癥通络药物能够减少ECM的积聚,可能是其防治肾小球硬化的机制之一。Objective To investigate the inhibitory effect of herb of supplement qi and nourish yin eliminating mass and dredge nels and collaterals on the mesangial cells （MCs） secreting extracellular matrix （ECM）.Methods Based on the method of culturing MCs in vitro and serum pharmacology,the contents of fibronectin （FN）,laminin （LN） and collagen Ⅳ （Col Ⅳ） in ECM were detected by ELISA.Results The contents of FN,LN,Col Ⅳ in ECM were all increased,while inhibited by herb of supplement qi and nourish yin eliminating mass and dredge nels and collaterals significantly.Conclusions Herb of supplement qi and nourish yin eliminating mass and dredge nels and collaterals can decrease the accumulation of ECM,which may be one of the mechanisms of preventing glomerulosclerosis.

关 键 词：系膜细胞 益气养阴、消癥通络 层黏连蛋白 纤维连接蛋白 Ⅳ型胶原 细胞外基质 

分 类 号：R285.6[医药卫生—中药学]