肉苁蓉总苷对β淀粉样蛋白25-35诱导PC12细胞凋亡的保护作用  

Protective effects of glycosides of cistanche on apoptosis of PC12 cells induced by aggregated β-amyloid protein 25-35

作　　者：罗兰[1] 阿尔孜古丽.吐尔逊 王晓雯[3] 

机构地区：[1]新疆医科大学第一附属医院药剂科,新疆乌鲁木齐830054 [2]新疆医科大学第一附属医院医学研究中心,新疆乌鲁木齐830054 [3]新疆医科大学药学院药理教研室,新疆乌鲁木齐830054

出　　处：《中国新药与临床杂志》2010年第2期115-118,共4页Chinese Journal of New Drugs and Clinical Remedies

基　　金：新疆维吾尔自治区自然科学基金项目(200121112)

摘　　要：目的研究肉苁蓉总苷(GCs)对β淀粉样蛋白25-35(Aβ25-35)诱导大鼠嗜铬细胞瘤PC12细胞凋亡的保护作用。方法体外培养的大鼠PC12细胞分6组:正常对照组、模型组、银杏叶片组(40mg·L-1)、GCs低剂量组(25mg·L-1)、GCs中剂量组(50mg·L-1)和GCs高剂量组(100mg·L-1)。细胞培养至对数生长期时分别加入上述各组药物,每日1次。培养h96时,模型组、银杏叶片组和GCs各剂量组分别加入终浓度20μmol·L-1的Aβ25-35,正常对照组以等体积培养基替代。继续培养24h和48h时,光镜观察各组细胞的形态及生长情况,MTT比色法检测各组细胞的存活率,分光光度法检测细胞中乳酸脱氢酶(LDH)活性,流式细胞术AnnexinⅤ/PI法检测细胞凋亡率。结果与正常对照组比较,模型组细胞数目显著减少,部分细胞皱缩,变圆、脱落的细胞数目较多,其存活率显著降低、LDH活性明显提高、凋亡率显著增加(P<0.01)。与模型组相比,GCs各剂量组细胞数目显著增多,细胞形态学变化明显改善,细胞的存活率增高、LDH活性下降、凋亡率降低,有非常显著差异(P<0.01)。其中,GCs高剂量作用48h效果最显著。结论GCs对Aβ25-35引起的PC12凋亡细胞损伤具有明显的保护作用。AIM To pheochromocytoma PC 12 cells were divided into six groups： dose GCs group （25 μg·L-1）, study the protective effects of glycosides of cistanche （GCs） on apoptosis in induced by aggregated β-amyloid protein 25-35（Aβ25-35）. METHODS PC12 cells normal control group, model group, Ginkgo biloba tablet group （40 mg·L-1）, low middle dose GCs group （50 mg·L-1）, and high dose GCs group （100 mg·L-1）.When PC12 cells entered log phase growth, the corresponding drugs were added to the culture medium once a day. Ninety-six hours later, Aβ25-35 （20μmol·L-1） were added into the model group, different dose GCs groups, and the Ginkgo biloba tablet group, while equal volume of medium was added into the normal control group. Twenty-four and forty-eight hours later, morphology and growth of the cells in all groups were observed by light microscope, survival rates were examined by MTY colorimetric assay, lactate dehydrogenase （LDH） activities were detected by the spectrophotometry, and the apoptotic rates were examined by Annexin V-FITC and staining with PI. RESULTS Compared with the normal control group, the cell number of the model group was reduced obviously, a part of cells became abscission and spherical in shape, the number of shrunken cells increased, the survival rate decreased, LDH activity increased and the apoptotic rate increased （P 〈 0.01）. Compared with the model group, the cell number of the GCs groups raised obviously, morphological change of the cell improved significantly, the survival rate increased, LDH activity reduced, and the apoptotic rate decreased （P 〈 0.01）. The change in high dose GCs group was the most remarkable at 48 h. CONCLUSION GCs has protective effects on apoptosis of PC12 cells induced by aggregated Aβ25-35.

关 键 词：肉苁蓉 PC12细胞 淀粉样Β蛋白 细胞凋亡 阿尔茨海默病 

分 类 号：R285[医药卫生—中药学] R965[医药卫生—中医学]