基于连接酶检测反应的并行分型系统检测鸡肉风味相关候选基因多态性  

A Typing System Based on Ligase Detection Reaction for Chicken Meat Flavor Related Candidate Genes Polymorphism

作　　者：李慧芳[1] 束婧婷[1] 张莹 邝智祥 朱文奇[1] 韩威[1] 陈宽维[1] 

机构地区：[1]中国农业科学院家禽研究所,扬州225003 [2]广东天农食品有限公司,清远511827

出　　处：《畜牧兽医学报》2010年第3期262-267,共6页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：现代农业产业技术体系建设专项资金;江苏省自然科学基金(BK2009177);江苏省家禽科学研究所青年(博士)基金(JQ2009001)

摘　　要：旨在建立一个基于聚合酶链反应—连接酶检测反应(LDR)的基因多态性并行检测系统,检测鸡肉风味相关候选基因脂肪型脂肪酸结合蛋白(A-FABP)基因、细胞外脂肪酸结合蛋白(Ex-FABP)基因和腺苷琥珀酸裂解酶(ADSL)基因的多态性,并分析其在优良地方鸡种——清远麻鸡和快大型隐性白羽鸡中的分布差异。采集165只隐性白羽鸡和185只清远麻鸡母鸡的血液并提取DNA,应用PCR-LDR方法检测A-FABP51C/T、Ex-FABP1011T/C和ADSL3484C/T的基因型。结果,所得分型结果同直接测序分型结果一致。3个多态位点在2个品种鸡中均表现为中度多态,并且在不同品种中的分布均存在差异,在A-FABP51C/T位点上达到显著水平。结果表明,建立的基于连接酶检测反应的基因多态性并行检测系统是一种准确、灵敏的SNP检测方案,为鸡肉风味性状相关基因的多态性研究提供了基础。To establish a parallel typing system based on ligase detection reaction （LDR） detecting the polymorphisms of A-FABP, Ex-FABP and ADSL genes, which were all candidate genes for chicken meat flavor, and to analyze the distribution differences of different genotypes in Qingyuan partridge chickens and Recessive White chickens, 165 hen samples and 185 hen samples were collected from Recessive White chicken and Qingyuan partridge chicken, respectively. The genotypes of A-FABP 51C/T, Ex-FABP 1011T/C and ADSL 3484C/T were identified by using PCR-LDR. The results by this method were coincidence with direct sequencing. Three SNPs all exhibited medium polymorphism. The allele distribution in different breeds was different, and the difference in A-FABP 51C/T locus was significant. The results indicated that the parallel typing system based on ligase detection reaction is a accurate and sensitive method to detect SNPs, and will lay the research foundation for the polymorphisms of candidate genes relating to meat flavor.

关 键 词：连接酶检测反应 A-FABP基因 Ex-FABP基因 ADSL基因 基因多态性 

分 类 号：S831.2[农业科学—畜牧学] Q343.13[农业科学—畜牧兽医]