朊蛋白在宁夏滩羊不同组织中表达的免疫组化研究  

Immunohistochemical Study on PrP Expression in Different Tissues of Tan Sheep in Ningxia

作　　者：许立华[1,2] 康晓东[3] 涂健[1] 张珠明[1,2] 杨建民[1] 周向梅[1] 尹晓敏[1] 杨利峰[1] 赵德明[1] 

机构地区：[1]中国农业大学动物医学院国家动物海绵状脑病实验室,北京100193 [2]宁夏大学农学院,银川750021 [3]宁夏农林科学院畜产工程技术中心,银川750004

出　　处：《畜牧兽医学报》2010年第3期301-304,共4页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：科技部星火计划(2006EA125015);国家科技支撑计划(2008BAI54B06)

摘　　要：作者运用免疫组织化学技术,首次对我国一类保护物种宁夏滩羊各组织中朊蛋白(PrP)的分布进行定性、定位研究。结果显示,PrP在滩羊的大脑、脑干、小脑、心脏、肝脏、脾脏、肾脏及淋巴结均有表达,尤其在脾脏、肾脏、脑干、淋巴结中的表达量丰富,而肺脏检测结果为阴性。此研究确定了朊蛋白在滩羊各组织中的表达与分布状况,并优化了朊蛋白检测的技术条件,为羊痒病的诊断与检测提供技术支持。In the present experiment, a qualitative detection was implemented to determine distri bution and location of prion protein（Prp） in different tissues of Ningxia Tan sheep, which is one of the protected species of class I . The result indicated that PrP has a wide range of expression in different tissues, such as cerebrum, brain stem, cerebellum, heart, liver, spleen, kidney, lymph node and so on. Especially, prion protein has a higher expression level in brain stem, spleen, kidney and lymph node than other tissues. We also got a negative result in the samples from lung. This study confirmed the status of distribution of PrP in different tissues from Tan sheep. In addition, the study provided a technical support to diagnose and detect scrapie by optimizing immunohistochemical method.

关 键 词：朊蛋白 组织 表达 免疫组化 滩羊 

分 类 号：S852.659.7[农业科学—基础兽医学]