中国H5N1亚型高致病性禽流感病毒抗原变异株的鉴定分析  

Identification of an Antigenic-drifted H5N1 Highly Pathogenic Virus in China

作　　者：李雁冰[1] 施建忠[1] 赵海丹[1] 田国彬[1] 王冬[1] 宋家升[1] 杨德全[1] 邓国华[1] 王秀荣[1] 陈化兰[1] 

机构地区：[1]中国农业科学院哈尔滨兽医研究所国家兽医生物技术重点开放实验室/国家禽流感参考实验室/农业部动物流感重点开放实验室,哈尔滨150001

出　　处：《畜牧兽医学报》2010年第3期305-309,共5页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：农业部动物流感监测与防制财政专项项目资助

摘　　要：对引起中国2006年山西、宁夏2省(区)H5N1亚型禽流感疫情的代表毒株——A/chicken/Shanxi/2/2006(H5N1)(CK/SX/06)进行了全面研究。结果表明该病毒具有高致病性禽流感病毒(HPAIV)特征,基因组序列分析发现其8个基因片段与中国传统H5N1亚型禽流感病毒GS/GD/1/96(H5N1)存在较大差异,属于新的基因型——山西鸡型;抗原性分析结果表明其在抗原性方面与GS/GD/1/96同样存在较大变异,将其命名为"山西鸡型"抗原变异株;以106EID50.0.1 mL-1剂量将该病毒经鼻腔接种4周龄SPF鸭以评价其对水禽的感染能力,结果表明其不感染鸭;常规方法接种BALB/c小鼠以评价其对哺乳动物的感染和致病能力,结果表明其能感染小鼠,但不引起死亡,呈低致病力。说明该类型高致病性H5N1 HPAIV目前仅危害鸡,不具备感染水禽的能力,感染哺乳动物但不致死。该类型病毒的出现与流行为中国禽流感的免疫与防制提出新的课题。In order to evaluate an avian influenza virus-CK/SX/2/06 （H5N1）, which was isolated from a poultry farm in Shanxi province. HA-HI test, RT-PCR, IVPI, antigenic analysis, se- quencing and phylogenic analysis, aquatic bird and BALB/c mice infection test were fulfilled in the study. The virus were HSN1 subtype highly pathogenic avian influenza virus （HPAIV）, which showed big diversity to reference strain GS/GD/1/96（HSN1） in China in phylogenic and antigenic analysis. So it was defined as antigenic-drifted strain, the same viruses established a new clade, Shanxi chicken-like strain, compared with the same subtype viruses which caused HSN1 HPAI outbreak in China in recent years. The virus didnPt infected SPF duck and showed low pathogenic to mammal. These results indicated that new clade and antigenic-drifted viruses Shanxi-like strain appeared in China, so we should adjust the measurement and strategy to prevent and control the disease.

关 键 词：H5N1 HPAIV CK/SX/2/06 山西鸡型 抗原变异 

分 类 号：S852.659.5[农业科学—基础兽医学]