去卵巢及雌激素替代治疗后家兔海马nNOS阳性神经元的形态结构及分布变化  

The Changes of Distribution and Structure of nNOS Positive Neurons in Rabbit Hippocampus after Ovariectomy and Estrogen Replacement Therapy

作　　者：谢有莲[1] 尹逊河[1] 邱建华[1] 赵军[1] 王宪龙[1] 

机构地区：[1]山东农业大学动物科技学院,泰安271018

出　　处：《畜牧兽医学报》2010年第3期329-334,共6页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：山东省自然科学基金资助项目(Y2003D04)

摘　　要：用SABC免疫组织化学技术,观察家兔海马各区nNOS阳性神经元在去卵巢及雌激素替代治疗后的形态结构及分布变化,为雌激素类药物防治绝经后老年性痴呆症提供理论依据。结果表明,家兔海马各区都有nNOS阳性神经元分布;去卵巢后海马nNOS阳性神经元的形态结构及分布变化有区域差异性:与假手术对照组相比,在海马CA1区、CA3区、齿状回(DG)阳性神经元数量明显减少(P<0.05),而在CA2区数量明显增多(P<0.05)。CA1、CA3区和DG的阳性神经元胞体截面积明显变小,最长突起长度明显变短,第一级突起数变少,与假手术组有显著差异(P<0.05)。CA2区阳性神经元胞体截面积明显变小(P<0.05),最长突起长度、第一级突起数增多,但差异不显著(P>0.05);nNOS阳性神经元的4种指标在雌激素替代治疗组与假手术组之间无显著差异(P>0.05)。结果提示:雌激素可能通过影响海马nNOS的表达来影响脑的学习和记忆功能。To study the related changes of nitric oxide synthase （nNOS） positive neurons in the sections of the hippocampus of female rabbit （Sprague-Dawley strain） after ovariectomy and estrogen replacement therapy, the distribution and structure of nNOS positive neurons was observed in rabbit hippocampus using SABC immunohistochemical technique, which provides basic theories for preventing and treating Alzheimerrs disease with estrogen replacement therapy. The results showed. There are comprehensive nNOS-positive staining cells in all regions of hippocampus. After ovariectomy, the changes of nNOS positive neurons in subregion of hippocampus were not equal, the number of positive neurons in subiculum CA1, CA3 and dentate gyrus（DG） were decreased compared with control group （P〈0.05）, while the number of positive neurons in subiculum CA2 was increased （P〈0.05）. The average section area of positive neuron was smaller and the length of the longest process become shorter and the number of first tuber turns were less in subiculum CA1, CA3 and DG than that in control group（P〈0.05）. The average section area of positive neuron was smaller while the length of the longest process and the number of first tuber have no remarkable change in CA2 than that in control group （P〉0.05）. Four kinds index of positive cells in the subregion of hippocampus showed no significant difference between estradiolreplacement therapy and the control group（P〉0.05）. The results indicated that estrogen maybe affect the learning and memory by influencing the expression of nNOS in hippocompus,

关 键 词：家兔 海马 神经型一氧化氮合酶 去卵巢 雌激素替代治疗 

分 类 号：S852.16[农业科学—基础兽医学]