阿维菌素对体外培养王鸽脑神经细胞凋亡和一氧化氮水平的影响  

Effects of Apoptosis and NO Level of AVM on King Pigeon Brain Cells in Vitro Culture

作　　者：李术[1] 崔雅莉[1] 王敏[1,2] 徐世文[1] 王俊杰[1] 

机构地区：[1]东北农业大学动物医学学院,哈尔滨150030 [2]黑龙江畜牧兽医职业学院,双城150100

出　　处：《畜牧兽医学报》2010年第3期347-352,共6页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家自然科学基金面上项目(30471278);黑龙江省教育厅科研项目(10541029)

摘　　要：本试验以阿维菌素染毒原代培养的王鸽脑神经细胞为模型,观察阿维菌素对神经细胞凋亡的毒性作用和一氧化氮产生量的影响。采用原位末端标记法观察细胞凋亡,四唑盐比色法检测神经细胞相对存活率,流式细胞术检测凋亡细胞百分比,分光光度法测定细胞一氧化氮的产生量。结果显示,阿维菌素可诱导神经细胞凋亡和细胞坏死,并具有随着阿维菌素剂量递增而增加的趋势,同时阿维菌素也能引起一氧化氮产生量降低,但是未发现存在剂量效应关系。The effects of apoptosis and NO level were explored with model of AVM on king pigeon brain neurocytes in vitro culture. Apoptosis was observed by TUNEL, the relative survival rate of neurocytes was detected by MTT, the percentage of apoptotic cells was detected by Flow cy- tometry, and the production of NO in cells was acquired by absorption spectrometry. The results showed that AVM can induce apoptosis and cytonecrosis of neurocyte, and has increased with the dose of AVM. AVM can reduced the production of NO, too, but no dose-response relationship.

关 键 词：阿维菌素 神经细胞 凋亡 一氧化氮 

分 类 号：S859.79[农业科学—临床兽医学] Q813.1[农业科学—兽医学]