受体母猪排卵状况对体细胞克隆胚胎移植效率的影响  

Effect of Ovulation Status on Efficiency of the Transplant in Recipient Gilts Transferred with Somatic Cloned Embryos

作　　者：罗学明[1,2] 张卫红[1,2] 冯冲[2] 龙川[2] 闫军[2] 赵会静[2] 张兆旺[1] 张子军[1,3] 潘登科[1,2] 

机构地区：[1]甘肃农业大学动物科学技术学院甘肃省草食动物生物技术重点实验室,兰州730070 [2]中国农业科学院北京畜牧兽医研究所农业部畜禽遗传资源与利用重点开放实验室,北京100193 [3]安徽农业大学动物科技学院,合肥230036

出　　处：《畜牧兽医学报》2010年第3期366-370,共5页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家"863"项目(2008AA101003)资助

摘　　要：为了确定猪体细胞克隆胚胎的受体母猪最佳发情阶段，本研究比较了供受体同期化方案对猪体细胞克隆胚胎输卵管内移植后30d受胎率和妊娠足月率的影响。将体外培养2d的猪体细胞克隆胚胎，移植到发情第1～2天的受体母猪。根据受体母猪卵泡发育与排卵状况将受体猪群分为2个类别：卵泡发育处于即将排卵或正在排卵阶段（组一）；卵泡发育处于排卵前阶段或完全排卵阶段（组二）。结果发现：（1）组一6头受体母猪胚胎移植后30d受胎率为100％；而组二10头受体母猪受胎率为40％，二者差异显著（P〈0．05）。（2）组一6头受体母猪全部产仔，妊娠足月率为100％；组二10头受胎母猪2头产仔，妊娠足月率为20％，二者差异极显著（P〈0．01）。以上结果表明：体外培养2d的猪体细胞克隆胚胎发育阶段与即将排卵或正在排卵的受体母猪的子宫环境同期性最好，得到了理想的胚胎移植效率。In this study, the effect of different ovulation status on pregnancy rates in surrogate recipient gilts receiving somatic nuclear transfer embryos was evaluated, in order to determine the optimal stage of the estrus cycle to achieve the highest pregnancy rates. Porcine somatic cell cloned embryos on 2 d in vitro were transplanted into the 1-2 d estrous recipient gilts. Recipient gilts were divided into two categories according to follicular development and ovulation status： just prior to ovulation or ovulating （Group 1）; per-ovulation or after ovulation（Group 2）. The results were as follows： （1） pregnancy rate on 30 d of six recipient gilts from Group 1 was 100%; and the pregnancy rate on 30 d of ten recipient gilts from Group 2 was 40%, the data significantly different between Group 1 and Group 2 （P〈0.05）; （2） For the full-term pregnancy rate of recip- ient gilts, extremely significant difference was observed between Group 1 and Group 2 （100% vs. 20%, P〈0. 01）. These results suggested that development stage of porcine somatic cloning embryos on 2 d in vitro with recipient giltls womb environment of ovulation status of just prior to ovulation or ovulating was the best in the same period. It may achieve the best efficiency of embryo transfer.

关 键 词：猪体细胞克隆 胚胎移植 排卵状况 受胎率 妊娠足月率 

分 类 号：S828.3[农业科学—畜牧学]